• Product name
  • Description
    Rabbit polyclonal to CDC45L
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 298-545 of Human CDC45L (NP_003495).

  • Positive control
    • H1299 whole cell lysate; A549 cells



Our Abpromise guarantee covers the use of ab97832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 66 kDa.
ICC/IF 1/100 - 1/200.


  • Function
    Required for initiation of chromosomal DNA replication.
  • Tissue specificity
    Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
  • Sequence similarities
    Belongs to the CDC45 family.
  • Developmental stage
    Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC 45 antibody
    • CDC 45L antibody
    • CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
    • CDC45 antibody
    • CDC45 cell division cycle 45 like antibody
    • CDC45 like antibody
    • CDC45 related protein antibody
    • CDC45_HUMAN antibody
    • CDC45L2 antibody
    • Cell division control protein 45 homolog antibody
    • Cell division cycle 45 like 2 antibody
    • Cell division cycle 45 like antibody
    • PORC PI 1 antibody
    • PORC-PI-1 antibody
    see all


  • Anti-CDC45L antibody (ab97832) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 66 kDa

    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A549 cells, using ab97832 at 1/200 dilution. Lower image is merged with DNA probe.


ab97832 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for contacting us.

I am sorry to hear of the issues which you have been experiencing when using ab97832 and would like to thank you for providing protocol details and the image. When looking at the datasheet for this product the western blot image shown does appear to have some non specific bands at about the same size as those you have been seeing with two near 50kDa and another faint band just above the specific band. However as you have mentioned, in your blot it is difficult to tell which band is specific as they both appear to have similar intensity. As such, I would like to recommend that you attempt to repeat this experiment with lower concentrations of ab97832 in attempt to titer the ideal concentration for your sample type/protocol. Reducing the antibodies concentration should have less effect on the specific band while reducing the non-specific band intensities. While we recommend a concentration range between 1:500- 1:3000, you may find that concentration beyond this range may give you the best results.

This product is covered by our Abpromise guarantee. If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, as you are using it according to specifications listed on our datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!

Read More


Sign up