Product nameAnti-CDC45L antibody [EPR5758]
See all CDC45L primary antibodies
DescriptionRabbit monoclonal [EPR5758] to CDC45L
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human CDC45L aa 50-150. The exact sequence is proprietary.
- HeLa, Jurkat, K562, and 293T cell lysates. Human kidney tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (KD)KD = 3.17 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).|
|IHC-P||1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol|
|ICC||1/50 - 1/100.|
|Flow Cyt||1/500 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRequired for initiation of chromosomal DNA replication.
Tissue specificityWidely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Sequence similaritiesBelongs to the CDC45 family.
Developmental stageTranscript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CDC 45 antibody
- CDC 45L antibody
- CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
All lanes : Anti-CDC45L antibody [EPR5758] (ab108350) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108350 at 1/50
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD