For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Rabbit monoclonal [EPR5758] to CDC45L
, Flow Cytmore detailsUnsuitable for:
Synthetic peptide within Human CDC45L aa 50-150. The exact sequence is proprietary.
- HeLa, Jurkat, K562, and 293T cell lysates. Human kidney tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (KD)
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
||1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol
||1/50 - 1/100.
||1/500 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IP.
Required for initiation of chromosomal DNA replication.
Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Belongs to the CDC45 family.
Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
Information by UniProt
- CDC 45 antibody
- CDC 45L antibody
- CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
Western blot - Anti-CDC45L antibody [EPR5758] (ab108350)
All lanes : Anti-CDC45L antibody [EPR5758] (ab108350) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC45L antibody [EPR5758] (ab108350)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108350 at 1/50
Other - Anti-CDC45L antibody [EPR5758] (ab108350)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"