Overview

  • Product name
    Anti-CDC45L antibody [EPR5759]
    See all CDC45L primary antibodies
  • Description
    Rabbit monoclonal [EPR5759] to CDC45L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CDC45L aa 500-600. The exact sequence is proprietary.

  • Positive control
    • 293T, Jurkat, and K562 cell lysates; Human colon tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.)
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Required for initiation of chromosomal DNA replication.
    • Tissue specificity
      Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
    • Sequence similarities
      Belongs to the CDC45 family.
    • Developmental stage
      Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDC 45 antibody
      • CDC 45L antibody
      • CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
      • CDC45 antibody
      • CDC45 cell division cycle 45 like antibody
      • CDC45 like antibody
      • CDC45 related protein antibody
      • CDC45_HUMAN antibody
      • CDC45L2 antibody
      • Cell division control protein 45 homolog antibody
      • Cell division cycle 45 like 2 antibody
      • Cell division cycle 45 like antibody
      • PORC PI 1 antibody
      • PORC-PI-1 antibody
      see all

    Images

    • ICC/IF image of ab126762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126762 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-CDC45L antibody [EPR5759] (ab126762) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size: 66 kDa

    • ab126762, at 1/100 dilution, staining CDC45L in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126762 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 14 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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