Product nameAnti-CDC45L antibody [EPR5759]
See all CDC45L primary antibodies
DescriptionRabbit monoclonal [EPR5759] to CDC45L
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CDC45L aa 500-600. The exact sequence is proprietary.
- 293T, Jurkat, and K562 cell lysates; Human colon tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.86 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.)|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionRequired for initiation of chromosomal DNA replication.
Tissue specificityWidely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Sequence similaritiesBelongs to the CDC45 family.
Developmental stageTranscript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- CDC 45 antibody
- CDC 45L antibody
- CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
ICC/IF image of ab126762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126762 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-CDC45L antibody [EPR5759] (ab126762) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 66 kDa
ab126762, at 1/100 dilution, staining CDC45L in paraffin-embedded Human colon tissue by Immunohistochemistry.
Equilibrium disassociation constant (KD)
Learn more about KD
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ab126762 has not yet been referenced specifically in any publications.