Recombinant Anti-CDC45L antibody [EPR5759] (ab126762)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5759] to CDC45L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CDC45L antibody [EPR5759]
See all CDC45L primary antibodies -
Description
Rabbit monoclonal [EPR5759] to CDC45L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CDC45L aa 500-600. The exact sequence is proprietary.
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Positive control
- 293T, Jurkat, and K562 cell lysates; Human colon tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.86 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5759 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.)
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ICC/IF | (1) |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 66 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.) |
ICC/IF
1/100 - 1/250. |
Target
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Function
Required for initiation of chromosomal DNA replication. -
Tissue specificity
Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. -
Sequence similarities
Belongs to the CDC45 family. -
Developmental stage
Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8318 Human
- Entrez Gene: 12544 Mouse
- Entrez Gene: 287961 Rat
- Omim: 603465 Human
- SwissProt: O75419 Human
- SwissProt: Q9Z1X9 Mouse
- Unigene: 474217 Human
- Unigene: 1248 Mouse
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Alternative names
- CDC 45 antibody
- CDC 45L antibody
- CDC45 (cell division cycle 45, S.cerevisiae homolog) like antibody
see all
Images
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ICC/IF image of ab126762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126762 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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All lanes : Anti-CDC45L antibody [EPR5759] (ab126762) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 66 kDa -
ab126762, at 1/100 dilution, staining CDC45L in paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab126762 has been referenced in 5 publications.
- Li GS et al. BCAT1: A risk factor in multiple cancers based on a pan-cancer analysis. Cancer Med 11:1396-1412 (2022). PubMed: 34984849
- Tan TCJ et al. Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8+ T lymphocytes. Nat Commun 13:7796 (2022). PubMed: 36528626
- Lu HP et al. Expression of Cell Division Cycle Protein 45 in Tissue Microarrays and the CDC45 Gene by Bioinformatics Analysis in Human Hepatocellular Carcinoma and Patient Outcomes. Med Sci Monit 27:e928800 (2021). PubMed: 33622998
- Huang J et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27:1794-1808.e5 (2019). PubMed: 31067464
- Li X et al. Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication. Mol Cell 72:650-660.e8 (2018). PubMed: 30392930