Recombinant Anti-CDC5L antibody [EPR7658] (ab129114)
![Western blot - Anti-CDC5L antibody [EPR7658] (ab129114)](https://www.abcam.com/ps/products/129/ab129114/Images/ab129114-143124-anti-cdc5l-antibody-epr7658-western-blot.jpg "Western blot - Anti-CDC5L antibody [EPR7658] (ab129114)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7658] to CDC5L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-CDC5L antibody [EPR7658]
See all CDC5L primary antibodies -
Description
Rabbit monoclonal [EPR7658] to CDC5L -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues in Human CDC5L (Q99459).
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Positive control
- Human kidney tissue; HepG2 cells; fetal brain and HeLa whole cell lysate (ab150035).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7658 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
|
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| ICC/IF |
1/250 - 1/500.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/250 - 1/500. |
Target
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Function
DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. -
Tissue specificity
Ubiquitously expressed in both fetal and adult tissues. -
Involvement in disease
A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2. -
Sequence similarities
Belongs to the CEF1 family.
Contains 2 HTH myb-type DNA-binding domains. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8. -
Cellular localization
Nucleus. Nucleus speckle. Cytoplasm. May shuttle between cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 988 Human
- Entrez Gene: 71702 Mouse
- Entrez Gene: 85434 Rat
- Omim: 602868 Human
- SwissProt: Q99459 Human
- SwissProt: Q6A068 Mouse
- SwissProt: O08837 Rat
- Unigene: 485471 Human
see all -
Alternative names
- CDC5 antibody
- CDC5 cell division cycle 5-like (S. pombe) antibody
- Cdc5 related protein antibody
see all
Images
-
Western blot - Anti-CDC5L antibody [EPR7658] (ab129114)All lanes : Anti-CDC5L antibody [EPR7658] (ab129114) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (ab129114)](https://www.abcam.com/ps/products/129/ab129114/Images/ab129114-143123-anti-cdc5l-antibody-epr7658-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (ab129114)ab129114, at a 1/250 dilution, staining CDC5L in HepG2 cells by immunofluorescence. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (ab129114)](https://www.abcam.com/ps/products/129/ab129114/Images/ab129114-143122-anti-cdc5l-antibody-epr7658-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (ab129114)ab129114, at a 1/250 dilution, staining CDC5L in paraffin-embedded Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-CDC5L antibody [EPR7658] (ab129114)](https://www.abcam.com/ps/products/129/ab129114/Images/ab129114-4-benefits-of-recombinant-antibodies.png)
Anti-CDC5L antibody [EPR7658] (ab129114)
Protocols
Datasheets and documents
References (0)
ab129114 has not yet been referenced specifically in any publications.
