Recombinant
RabMAb

Recombinant Anti-CDC5L antibody [EPR7658] (ab129114)

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Overview

  • Product name

    Anti-CDC5L antibody [EPR7658]
    See all CDC5L primary antibodies

  • Description

    Rabbit monoclonal [EPR7658] to CDC5L

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human CDC5L (Q99459).

  • Positive control

    • Human kidney tissue; HepG2 cells; fetal brain and HeLa whole cell lysate (ab150035).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7658

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

    • Tissue specificity

      Ubiquitously expressed in both fetal and adult tissues.

    • Involvement in disease

      A chromosomal aberration involving CDC5L is found in multicystic renal dysplasia. Translocation t(6;19)(p21;q13.1) with USF2.

    • Sequence similarities

      Belongs to the CEF1 family.
      Contains 2 HTH myb-type DNA-binding domains.

    • Post-translational
      modifications

      Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.

    • Cellular localization

      Nucleus. Nucleus speckle. Cytoplasm. May shuttle between cytoplasm and nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CDC5 antibody
      • CDC5 cell division cycle 5-like (S. pombe) antibody
      • Cdc5 related protein antibody
      • CDC5-LIKE antibody
      • Cdc5-like protein antibody
      • Cdc5l antibody
      • CDC5L_HUMAN antibody
      • CEF1 antibody
      • Cell division cycle 5 S. pombe homolog of antibody
      • Cell division cycle 5-like protein antibody
      • dJ319D22.1 antibody
      • KIAA0432 antibody
      • PCDC5RP antibody
      • Pombe Cdc5 related protein antibody
      • Pombe Cdc5-related protein antibody
      see all

    Images

    • Western blot - Anti-CDC5L antibody [EPR7658] (ab129114)
      Western blot - Anti-CDC5L antibody [EPR7658] (ab129114)
      All lanes : Anti-CDC5L antibody [EPR7658] (ab129114) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 92 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (ab129114)
      Immunocytochemistry/ Immunofluorescence - Anti-CDC5L antibody [EPR7658] (ab129114)
      ab129114, at a 1/250 dilution, staining CDC5L in HepG2 cells by immunofluorescence.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (ab129114)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC5L antibody [EPR7658] (ab129114)

      ab129114, at a 1/250 dilution, staining CDC5L in paraffin-embedded Human kidney tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab129114 has not yet been referenced specifically in any publications.

    Publishing research using ab129114? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    What is the classification of this antibody, RUO, ASR, or IVD?

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    Answer

    All of Abcam's antibodies are RUO. The only ones that are IVD are the ones we offer through CellMarque. I've checked and unfortunately we do not have an anti-CDC5L for IVD. http://shop.cellmarque.com/antibodies/Epitomics-Portfolio.asp

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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