Overview

  • Product name
  • Description
    Rabbit polyclonal to Cdc6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 27-372 of Human Cdc6 (Uniprot ID: Q99741).

  • Positive control
    • Jurkat, Neuro2A, GL261, C8D30, BCL-1, Raw264.7, C2C12, PC-12 and Rat2 whole cell lysates; A431 cells.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 63 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
  • Sequence similarities
    Belongs to the CDC6/cdc18 family.
  • Cellular localization
    Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc 18L antibody
    • Cdc 6 antibody
    • CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
    • CDC18 (S.pombe) antibody
    • Cdc18 related protein antibody
    • CDC18(S.pombe) antibody
    • Cdc18-related protein antibody
    • Cdc18L antibody
    • cdc6 antibody
    • CDC6 cell division cycle 6 homolog antibody
    • CDC6 related protein antibody
    • CDC6-related protein antibody
    • CDC6_HUMAN antibody
    • Cdc6p antibody
    • CELL CYCLE CONTROLLER CDC6 antibody
    • Cell division control protein 6 antibody
    • Cell division control protein 6 homolog antibody
    • Cell division cycle 6 homolog (S. cerevisiae) antibody
    • Cell division cycle 6 homolog antibody
    • Cell division cycle 6, S. cerevisiae, homolog of antibody
    • HsCDC 6 antibody
    • HsCDC18 antibody
    • HsCDC6 antibody
    • p62 antibody
    • p62(cdc 6) antibody
    • p62(cdc6) antibody
    see all

Images

  • Anti-Cdc6 antibody (ab155759) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 63 kDa



    7.5% SDS PAGE
  • All lanes : Anti-Cdc6 antibody (ab155759) at 1/1000 dilution

    Lane 1 : Neuro2A whole cell
    lysate/extract
    Lane 2 : GL261 whole cell
    lysate/extract
    Lane 3 : C8D30 whole cell
    lysate/extract
    Lane 4 : BCL-1 whole cell
    lysate/extract
    Lane 5 : Raw264.7 whole cell
    lysate/extract
    Lane 6 : C2C12 whole cell
    lysate/extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 63 kDa



    7.5% SDS-PAGE
  • All lanes : Anti-Cdc6 antibody (ab155759) at 1/1000 dilution

    Lane 1 : PC-12 whole cell
    lysate/extract
    Lane 2 : Rat2 whole cell
    lysate/extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 63 kDa



    7.5% SDS-PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labeling Cdc6 with ab155759 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).

References

ab155759 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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