Key features and details
- Rabbit polyclonal to Cdc6
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cdc6 antibody
See all Cdc6 primary antibodies
DescriptionRabbit polyclonal to Cdc6
Tested applicationsSuitable for: IP, IHC-Pmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human Cdc6 aa 1-50. The exact sequence is proprietary. (NP_001245.1).
Database link: Q99741
- IP: HeLa whole cell lysate. IHC-P: Human lung carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245446 was affinity purified using an epitope specific to Cdc6 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Sequence similaritiesBelongs to the CDC6/cdc18 family.
Cellular localizationNucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
- Information by UniProt
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Cdc6 with ab245446 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
Cdc6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) with ab245446 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative anti-Cdc6 antibody at 1 µg/ml.
Lane 1: ab245446 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245446 has not yet been referenced specifically in any publications.