Anti-Cdc6 antibody [Cdc6 9H8/5] (ab20150)
Key features and details
- Mouse monoclonal [Cdc6 9H8/5] to Cdc6
- Suitable for: IP, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-Cdc6 antibody [Cdc6 9H8/5]
See all Cdc6 primary antibodies -
Description
Mouse monoclonal [Cdc6 9H8/5] to Cdc6 -
Host species
Mouse -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Full length protein corresponding to Saccharomyces cerevisiae Cdc6.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
Cdc6 9H8/5 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| Notes |
|---|
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
Target
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Function
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. -
Sequence similarities
Belongs to the CDC6/cdc18 family. -
Cellular localization
Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells. - Information by UniProt
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Alternative names
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab20150 has been referenced in 6 publications.
- Evrin C et al. In the absence of ATPase activity, pre-RC formation is blocked prior to MCM2-7 hexamer dimerization. Nucleic Acids Res 41:3162-72 (2013). WB . PubMed: 23376927
- Evrin C et al. The ORC/Cdc6/MCM2-7 complex facilitates MCM2-7 dimerization during prereplicative complex formation. Nucleic Acids Res N/A:N/A (2013). IP . PubMed: 24234446
- Ikui AE et al. A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication. PLoS Genet 8:e1003099 (2012). WB . PubMed: 23236290
- Zhang JY et al. Tumor necrosis factor receptor 1/c-Jun-NH2-kinase signaling promotes human neoplasia. Cancer Res 67:3827-34 (2007). WB . PubMed: 17440097
- Perkins G et al. Separate SCF(CDC4) recognition elements target Cdc6 for proteolysis in S phase and mitosis. EMBO J 20:4836-45 (2001). PubMed: 11532947
- Donovan S et al. Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast. Proc Natl Acad Sci U S A 94:5611-6 (1997). PubMed: 9159120
