Overview

  • Product name

    Anti-Cdc6 antibody [EPR714(2)]
    See all Cdc6 primary antibodies
  • Description

    Rabbit monoclonal [EPR714(2)] to Cdc6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cdc6 aa 1-100 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab197759)

  • Positive control

    • WB: Hela, 293T, HCT116 treated with hydroxyurea and Raji treated with FBS lysates IHC-P: Human gastric adenocarcinoma, breast carcinoma, thyroid cancer tissue ICC/IF: HeLa FC: HeLa
  • General notes

     

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

For unpurified use at 1/250 - 1/500

WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/90. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt 1/90.

Target

  • Function

    Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
  • Sequence similarities

    Belongs to the CDC6/cdc18 family.
  • Cellular localization

    Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cdc 18L antibody
    • Cdc 6 antibody
    • CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
    • CDC18 (S.pombe) antibody
    • Cdc18 related protein antibody
    • CDC18(S.pombe) antibody
    • Cdc18-related protein antibody
    • Cdc18L antibody
    • cdc6 antibody
    • CDC6 cell division cycle 6 homolog antibody
    • CDC6 related protein antibody
    • CDC6-related protein antibody
    • CDC6_HUMAN antibody
    • Cdc6p antibody
    • CELL CYCLE CONTROLLER CDC6 antibody
    • Cell division control protein 6 antibody
    • Cell division control protein 6 homolog antibody
    • Cell division cycle 6 homolog (S. cerevisiae) antibody
    • Cell division cycle 6 homolog antibody
    • Cell division cycle 6, S. cerevisiae, homolog of antibody
    • HsCDC 6 antibody
    • HsCDC18 antibody
    • HsCDC6 antibody
    • p62 antibody
    • p62(cdc 6) antibody
    • p62(cdc6) antibody
    see all

Images

  • All lanes : Anti-Cdc6 antibody [EPR714(2)] (ab109315) at 0.5 µg/ml (purified)

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : 293T (Human embryonic kidney epithelial cell) treated with 20mM Hydtoxyurea for 24 hours whole cell lysates
    Lane 3 : HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates
    Lane 4 : HCT116 (Human colorectal carcinoma epithelial cell) treated with 1mM Hydtoxyurea for 16 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

    Hydroxyurea induces Cdc6 expression (PMID:11346650)

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cdc6 with Purified ab109315 at 1:100 dilution (9.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling Cdc6 with Purified ab109315 at 1:90 dilution (10.4 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
  • Immunohistochemical analysis of Cdc6 in paraffin embedded Human gastric adenocarcinoma tissue, using unpurified ab109315 at a 1/1000 dilution.

  • Immunohistochemical analysis of Cdc6 in paraffin embedded Human breast carcinoma tissue, using unpurified ab109315 at a 1/1000 dilution.

  • Unpurified ab109315 staining Cdc6 in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. Ab150081 (1/200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Hu W  et al. MiR-373-3p enhances the chemosensitivity of gemcitabine through cell cycle pathway by targeting CCND2 in pancreatic carcinoma cells. Biomed Pharmacother 105:887-898 (2018). Read more (PubMed: 30021382) »
  • Zhang K  et al. Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis. Cell Death Dis 9:1035 (2018). Read more (PubMed: 30305611) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 23 2017

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