Recombinant Anti-Cdc6 antibody [EPR714(2)] (ab109315)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-Cdc6 antibody [EPR714(2)]
See all Cdc6 primary antibodies -
Description
Rabbit monoclonal [EPR714(2)] to Cdc6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cdc6 aa 1-100 (N terminal). The exact sequence is proprietary.
(Peptide available asab197759) -
Positive control
- WB: Hela, 293T, HCT116 treated with hydroxyurea and Raji treated with FBS lysates IHC-P: Human gastric adenocarcinoma, breast carcinoma, thyroid cancer tissue ICC/IF: HeLa FC: HeLa
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR714(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. For unpurified use at 1/250 - 1/500 |
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| WB | 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 63 kDa). | |
| IHC-P | 1/90. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/90. |
Target
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Function
Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. -
Sequence similarities
Belongs to the CDC6/cdc18 family. -
Cellular localization
Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells. - Information by UniProt
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Database links
- Entrez Gene: 990 Human
- Omim: 602627 Human
- SwissProt: Q99741 Human
- Unigene: 405958 Human
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Alternative names
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
see all
Images
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![Western blot - Anti-Cdc6 antibody [EPR714(2)] (ab109315)](https://www.abcam.com/ps/products/109/ab109315/Images/ab109315-314261-anti-cdc6-antibody-epr7142-western-blot.jpg)
Western blot - Anti-Cdc6 antibody [EPR714(2)] (ab109315)All lanes : Anti-Cdc6 antibody [EPR714(2)] (ab109315) at 0.5 µg/ml (purified)
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : 293T (Human embryonic kidney epithelial cell) treated with 20mM Hydtoxyurea for 24 hours whole cell lysates
Lane 3 : HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates
Lane 4 : HCT116 (Human colorectal carcinoma epithelial cell) treated with 1mM Hydtoxyurea for 16 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Hydroxyurea induces Cdc6 expression (PMID:11346650) -
![Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (ab109315)](https://www.abcam.com/ps/products/109/ab109315/Images/ab109315-314328-anti-cdc6-antibody-epr7142-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (ab109315)Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cdc6 with Purified ab109315 at 1:100 dilution (9.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)](https://www.abcam.com/ps/products/109/ab109315/Images/ab109315-314301-anti-cdc6-antibody-epr7142-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling Cdc6 with Purified ab109315 at 1:90 dilution (10.4 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)](https://www.abcam.com/ps/products/109/ab109315/Images/ab109315-122084-anti-cdc6-antibody-epr7142-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)Immunohistochemical analysis of Cdc6 in paraffin embedded Human gastric adenocarcinoma tissue, using unpurified ab109315 at a 1/1000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)](https://www.abcam.com/ps/products/109/ab109315/Images/ab109315-122083-anti-cdc6-antibody-epr7142-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)Immunohistochemical analysis of Cdc6 in paraffin embedded Human breast carcinoma tissue, using unpurified ab109315 at a 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (ab109315)](https://www.abcam.com/ps/products/109/ab109315/Images/ab109315-300217-anti-cdc6-antibody-epr7142-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (ab109315)This image is courtesy of an abreview by Kirk McmanusUnpurified ab109315 staining Cdc6 in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. Ab150081 (1/200) was used as the secondary antibody.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Hu W et al. MiR-373-3p enhances the chemosensitivity of gemcitabine through cell cycle pathway by targeting CCND2 in pancreatic carcinoma cells. Biomed Pharmacother 105:887-898 (2018). Read more (PubMed: 30021382) »
- Zhang K et al. Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis. Cell Death Dis 9:1035 (2018). Read more (PubMed: 30305611) »
