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RecombinantRabMAb

Recombinant Anti-Cdc6 antibody [EPR714(2)] (ab109315)

  • Datasheet
Reviews (1) Submit a question References (11)

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Western blot - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
  • Anti-Cdc6 antibody [EPR714(2)] (ab109315)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR714(2)] to Cdc6
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Cdc6 antibody [EPR714(2)]
    See all Cdc6 primary antibodies
  • Description

    Rabbit monoclonal [EPR714(2)] to Cdc6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt (Intra)
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Hela, 293T, HCT116 treated with hydroxyurea and Raji treated with FBS lysates IHC-P: Human gastric adenocarcinoma, breast carcinoma, thyroid cancer tissue ICC/IF: HeLa
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR714(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Associated products

  • Alternative Versions

    • Anti-Cdc6 antibody [EPR714(2)] - BSA and Azide free (ab211734)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/100.

For unpurified use at 1/250 - 1/500

WB
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 63 kDa).
IHC-P
1/90. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
ICC/IF
1/100.

For unpurified use at 1/250 - 1/500

WB
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 63 kDa).
IHC-P
1/90. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Application notes
Is unsuitable for Flow Cyt (Intra).

Target

  • Function

    Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
  • Sequence similarities

    Belongs to the CDC6/cdc18 family.
  • Cellular localization

    Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
  • Target information above from: UniProt accession Q99741 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 990 Human
    • Omim: 602627 Human
    • SwissProt: Q99741 Human
    • Unigene: 405958 Human
    • Alternative names

      • Cdc 18L antibody
      • Cdc 6 antibody
      • CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
      • CDC18 (S.pombe) antibody
      • Cdc18 related protein antibody
      • CDC18(S.pombe) antibody
      • Cdc18-related protein antibody
      • Cdc18L antibody
      • cdc6 antibody
      • CDC6 cell division cycle 6 homolog antibody
      • CDC6 related protein antibody
      • CDC6-related protein antibody
      • CDC6_HUMAN antibody
      • Cdc6p antibody
      • CELL CYCLE CONTROLLER CDC6 antibody
      • Cell division control protein 6 antibody
      • Cell division control protein 6 homolog antibody
      • Cell division cycle 6 homolog (S. cerevisiae) antibody
      • Cell division cycle 6 homolog antibody
      • Cell division cycle 6, S. cerevisiae, homolog of antibody
      • HsCDC 6 antibody
      • HsCDC18 antibody
      • HsCDC6 antibody
      • p62 antibody
      • p62(cdc 6) antibody
      • p62(cdc6) antibody
      see all

    Images

    • Western blot - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
      Western blot - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
      All lanes : Anti-Cdc6 antibody [EPR714(2)] (ab109315) at 0.5 µg/ml (purified)

      Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : 293T (Human embryonic kidney epithelial cell) treated with 20mM Hydroxyurea for 24 hours whole cell lysates
      Lane 3 : HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates
      Lane 4 : HCT116 (Human colorectal carcinoma epithelial cell) treated with 1mM Hydtoxyurea for 16 hours whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 63 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

      Hydroxyurea induces Cdc6 expression (PMID:11346650)

    • Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
      Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cdc6 with Purified ab109315 at 1:100 dilution (9.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling Cdc6 with Purified ab109315 at 1:90 dilution (10.4 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)

      Immunohistochemical analysis of Cdc6 in paraffin embedded Human gastric adenocarcinoma tissue, using unpurified ab109315 at a 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc6 antibody [EPR714(2)] (ab109315)

      Immunohistochemical analysis of Cdc6 in paraffin embedded Human breast carcinoma tissue, using unpurified ab109315 at a 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (ab109315)
      Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody [EPR714(2)] (ab109315)This image is courtesy of an abreview by Kirk Mcmanus

      Unpurified ab109315 staining Cdc6 in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. Ab150081 (1/200) was used as the secondary antibody.

      See Abreview

    • Anti-Cdc6 antibody [EPR714(2)] (ab109315)
      Anti-Cdc6 antibody [EPR714(2)] (ab109315)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (11)

    Publishing research using ab109315? Please let us know so that we can cite the reference in this datasheet.

    ab109315 has been referenced in 11 publications.

    • Yicong Y  et al. Increased CDC6 Expression Associates With Poor Prognosis in Patients With Clear Cell Renal Cell Carcinoma. Front Oncol 11:666418 (2021). PubMed: 34136398
    • Ke Y  et al. RYBP inhibits esophageal squamous cell carcinoma proliferation through downregulating CDC6 and CDC45 in G1-S phase transition process. Life Sci 250:117578 (2020). PubMed: 32209426
    • Zhang X  et al. Long-Noncoding RNA CASC9 Promotes Progression of Non-Small Cell Lung Cancer by Promoting the Expression of CDC6 Through Binding to HuR. Cancer Manag Res 12:9033-9043 (2020). PubMed: 33061598
    • Cai J  et al. The RNA-Binding Protein HuR Confers Oxaliplatin Resistance of Colorectal Cancer By Upregulating CDC6. Mol Cancer Ther 18:1243-1254 (2019). PubMed: 31064870
    • Iwai K  et al. Molecular mechanism and potential target indication of TAK-931, a novel CDC7-selective inhibitor. Sci Adv 5:eaav3660 (2019). PubMed: 31131319
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cdc6 antibody [EPR714(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    DR. Kirk Mcmanus

    Verified customer

    Submitted Aug 23 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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