Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) preadsorbed
Additional data
Additional Notes
Asynchronous HeLa cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Under these conditions, this antibody produces a nuclear-enriched, focal staining pattern that is enriched in a subset of interphase nuclei (as expected). MeOH-fixed samples were also evaluated and produce a similar staining pattern.
DR. Kirk Mcmanus
Verified customer
Submitted Aug 23 2017