Recombinant
RabMAb

Recombinant Anti-Cdc6 antibody [EPR714(2)] - BSA and Azide free (ab211734)

Overview

  • Product name

    Anti-Cdc6 antibody [EPR714(2)] - BSA and Azide free
    See all Cdc6 primary antibodies
  • Description

    Rabbit monoclonal [EPR714(2)] to Cdc6 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cdc6 aa 1-100 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab197759)

  • Positive control

    • WB: Hela treated with hydroxyurea and Raji treated with FBS lysates IHC-P: Human gastric adenocarcinoma and Human breast carcinoma tissue
  • General notes

    ab211734 is the carrier-free version of ab109315 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab211734 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 62 kDa (predicted molecular weight: 63 kDa).
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Perform antigen retrieval before commencing with IHC staining protocol.

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
    • Sequence similarities

      Belongs to the CDC6/cdc18 family.
    • Cellular localization

      Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cdc 18L antibody
      • Cdc 6 antibody
      • CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
      • CDC18 (S.pombe) antibody
      • Cdc18 related protein antibody
      • CDC18(S.pombe) antibody
      • Cdc18-related protein antibody
      • Cdc18L antibody
      • cdc6 antibody
      • CDC6 cell division cycle 6 homolog antibody
      • CDC6 related protein antibody
      • CDC6-related protein antibody
      • CDC6_HUMAN antibody
      • Cdc6p antibody
      • CELL CYCLE CONTROLLER CDC6 antibody
      • Cell division control protein 6 antibody
      • Cell division control protein 6 homolog antibody
      • Cell division cycle 6 homolog (S. cerevisiae) antibody
      • Cell division cycle 6 homolog antibody
      • Cell division cycle 6, S. cerevisiae, homolog of antibody
      • HsCDC 6 antibody
      • HsCDC18 antibody
      • HsCDC6 antibody
      • p62 antibody
      • p62(cdc 6) antibody
      • p62(cdc6) antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cdc6 with Purified ab109315 at 1:100 dilution (9.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109315).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling Cdc6 with Purified ab109315 at 1:90 dilution (10.4 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109315).

    • Unpurified ab109315 staining Cdc6 in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. Ab150081 (1/200) was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109315).

    • Immunohistochemical analysis of Cdc6 in paraffin embedded Human gastric adenocarcinoma tissue, using unpurified ab109315 at a 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109315).

    • Immunohistochemical analysis of Cdc6 in paraffin embedded Human breast carcinoma tissue, using unpurified ab109315 at a 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109315).

    References

    ab211734 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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