Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR761(2)] to Cdc6 (phospho S106) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-Cdc6 (phospho S106) antibody [EPR761(2)] - BSA and Azide free
See all Cdc6 primary antibodies
DescriptionRabbit monoclonal [EPR761(2)] to Cdc6 (phospho S106) - BSA and Azide free
The antibody only detects Cdc6 phosphorylated on Serine 106.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Cdc6 (phospho S106). The exact sequence is proprietary.
ab247471 is the carrier-free version of ab76422 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247471 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab247471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).Can be blocked with Cdc6 (phospho S106) peptide (ab197758).|
FunctionInvolved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Sequence similaritiesBelongs to the CDC6/cdc18 family.
Cellular localizationNucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
- Information by UniProt
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
All lanes : Anti-Cdc6 (phospho S106) antibody [EPR761(2)] (ab76422) at 1/2000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Raji cell lysate treated with FBS
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
This data was developed using ab76422, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247471 has not yet been referenced specifically in any publications.