Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR759Y] to Cdc6 (phospho S54)
- Suitable for: WB, ICC/IF, Dot blot
- Reacts with: Human
Product nameAnti-Cdc6 (phospho S54) antibody [EPR759Y]
See all Cdc6 primary antibodies
DescriptionRabbit monoclonal [EPR759Y] to Cdc6 (phospho S54)
Specificityab75809 recognises Cdc6 only when phosphorylated at Serine 54.
Tested applicationsSuitable for: WB, ICC/IF, Dot blotmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
A phospho-specific peptide corresponding to residues surrounding serine 54 of human Cdc6
- Raji + FBS cell lysates. HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).|
|ICC/IF||1/100 - 1/250.|
FunctionInvolved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Sequence similaritiesBelongs to the CDC6/cdc18 family.
Cellular localizationNucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
- Information by UniProt
- Cdc 18L antibody
- Cdc 6 antibody
- CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/5000 dilution
Lane 1 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Hela (Human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h). Whole cell lysates
Lane 3 : Hela (Human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h). Whole cell lysates.Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Exposure time: 30 seconds
Blocking buffer and diluting buffer: 5% NFDM/TBST
Primary antibody dilution 1:1000 dilution. Secondary antibody used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM /TBST. Lane 1:Cdc6 (pS54) phospho peptide, Lane 2:Cdc6 non-phospho peptide. Exposure time 3 minutes
Cell line 293T (Human embryonic kidney epithelial cell). Target AbID Ab75809 anti- Cdc6 (phospho S54)
ab150077 AlexaFluor®488 Goat anti-Rabbit secondary. Counterstain AbID Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Fixative 4% Paraformaldehyde. Permeabilisation 0.1% tritonX-100. Nuclear counter stain DAPI. Datasheet Comments Confocal image showing the expression was increased after treatment with Hydroxyurea (3mM 20hour), the signal was decreased after treatment with the Lambda Protein Phosphatase 31℃ for 2h. Target 1oAb dilution 1:100 2.4 μg/ml, Target 2ndry Ab dilution 1:1000 2 μg/ml, Counterstain Ab dilution 1:200 2.5 μg/ml
All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/2000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Raji +
FBS cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
ab75809 has been referenced in 4 publications.
- Zhang J et al. BRD4 facilitates replication stress-induced DNA damage response. Oncogene 37:3763-3777 (2018). PubMed: 29636547
- Müllers E et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). PubMed: 28345297
- Karanika S et al. Targeting DNA Damage Response in Prostate Cancer by Inhibiting Androgen Receptor-CDC6-ATR-Chk1 Signaling. Cell Rep 18:1970-1981 (2017). PubMed: 28228262
- Fan X et al. Role of Cdc6 in re-replication in cells expressing human papillomavirus E7 oncogene. Carcinogenesis 37:799-809 (2016). PubMed: 27207654