Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4730] to CDC7
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-CDC7 antibody [EPR4730]
See all CDC7 primary antibodies
DescriptionRabbit monoclonal [EPR4730] to CDC7
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CDC7 aa 1-100 (internal sequence). The exact sequence is proprietary.
- Jurkat, HeLa, and PC3 cell lysates; Jurkat cells.
Previously labelled as Cdc7 Kinase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSeems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- Cdc 7 antibody
- CDC7 antibody
- CDC7 cell division cycle 7 antibody
All lanes : Anti-CDC7 antibody [EPR4730] (ab108332) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa
ab108332, at 1/100 dilution, staining CDC7 in permeabilized Jurkat cells by Flow cytometry (red). Rabbit IgG (green).
ab108332 has been referenced in 3 publications.
- Li Q et al. CDC7-dependent transcriptional regulation of RAD54L is essential for tumorigenicity and radio-resistance of glioblastoma. Transl Oncol 11:300-306 (2018). PubMed: 29413763
- Pérez-Olivares M et al. Functional interplay between c-Myc and Max in B lymphocyte differentiation. EMBO Rep 19:N/A (2018). PubMed: 30126925
- Zhen H et al. Toona Sinensis and Moschus Decoction Induced Cell Cycle Arrest in Human Cervical Carcinoma HeLa Cells. Evid Based Complement Alternat Med 2014:121276 (2014). WB ; Human . PubMed: 24511319