- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-CDCA2 antibody
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DescriptionRabbit polyclonal to CDCA2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human CDCA2.
(Peptide available as ab45267.)
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Positive control
- This antibody gave a positive control in the following human lysates: Hela nuclear HepG2 nuclear Jurkat nuclear (data not shown)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab45129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 113 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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RelevanceCDCA2 is a regulator of chromosome structure during mitosis. CDCA2 forms an essential complex with protein phosphatase PP1-gamma subunit 1 (PPP1CC) and is required for the recruitment of PP1 to chromatin. Knockdown of CDCA2 by RNAi causes large-scale cell death by apoptosis, as does overexpression of a dominant-negative mutant of the protein.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 157313 Human
- SwissProt: Q69YH5 Human
- Unigene: 33366 Human
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Alternative names
- cell division cycle associated 2 antibody
- PPP1R81 antibody
- protein phosphatase 1 [gamma] (PPP1R3A) binding protein Repo-Man antibody
see all
Images
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Western blot - Anti-CDCA2 antibody (ab45129)All lanes : Anti-CDCA2 antibody (ab45129) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 :HepG2 nuclear extract lysate (ab14660)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 113 kDa
Observed band size: 125 kDa (why is the actual band size different from the predicted?)
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-CDCA2 antibody (ab45129)ICC/IF image of ab45129 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Qian J et al. Cdk1 orders mitotic events through coordination of a chromosome-associated phosphatase switch. Nat Commun 6:10215 (2015). Read more (PubMed: 26674376) »
- Wurzenberger C et al. Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores. J Cell Biol 198:173-83 (2012). Read more (PubMed: 22801782) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"