Key features and details
- Rabbit polyclonal to CDCA3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CDCA3 antibody
See all CDCA3 primary antibodies
DescriptionRabbit polyclonal to CDCA3
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa whole cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to CDCA3 immobilized on solid support.
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- SCF Complex E3 Ligase
Our Abpromise guarantee covers the use of ab80407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Is unsuitable for WB. ab80407 has not performed satisfactorily when used for WB of CDCA3 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of CDCA3.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCDCA3 (Cell division cycle associated 3) is an F-box-like protein which is required for entry into mitosis. It is associated with SKP1 as part of a SCF (SKP1-cullin-F-box) protein ligase complex, and is required for the ubiquitination and degradation of the CDK1 inhibitory tyrosine kinase WEE1 at G2/M phase.
Cellular localizationCytoplasmic. Cytosol.
- C8 antibody
- CDCA 3 antibody
- Cell division cycle associated 3 antibody
Detection of human CDCA3/TOME1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. ab80407 was used for IP at 3 µg/mg lysate (Lane 1). Lane 2 represents the control IgG. For subsequent WB, 0.1 µg/ml of ab80407 was used. Detection: Chemiluminescence with an exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab80407 has not yet been referenced specifically in any publications.