Recombinant
RabMAb

Recombinant Anti-CDCA5 antibody [EPR16331] - C-terminal (Phycoerythrin) (ab225377)

Overview

  • Product name

    Anti-CDCA5 antibody [EPR16331] - C-terminal (Phycoerythrin)
    See all CDCA5 primary antibodies
  • Description

    Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CDCA5 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96FF9

  • Positive control

    • Flow Cyt: MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.
  • Sequence similarities

    Belongs to the sororin family.
  • Domain

    The KEN box is required for the association with the APC/C complex.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.
    Ubiquitinated by the APC/C complex in G1, leading to its degradation.
  • Cellular localization

    Nucleus. Chromosome. Cytoplasm. Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDCA5 antibody
    • CDCA5_HUMAN antibody
    • Cell division cycle associated protein 5 antibody
    • Cell division cycle-associated protein 5 antibody
    • MGC16386 antibody
    • p35 antibody
    • Sororin antibody
    see all

Images

  • Overlay histogram showing MCF7 cells stained with ab225377 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225377, 1/5000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in MCF7 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab225377 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab225377.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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