Key features and details
- Rabbit polyclonal to CDCA7L/HR1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-CDCA7L/HR1 antibody
See all CDCA7L/HR1 primary antibodies
DescriptionRabbit polyclonal to CDCA7L/HR1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: RT4 and NTERA-2 whole cell lysates. IHC-P: Human appendix tissue. ICC/IF: A431 cells.
This product was previously labelled as CDCA7L
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 52 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionPlays a role in transcriptional regulation as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to the promoter. Plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells. Involved in apoptotic signaling pathways; May act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1.
Tissue specificityUbiquitous. Overexpressed in medulloblastoma.
Cellular localizationCytoplasm. Nucleus. Associates with chromatin. Translocates from cytoplasm to nucleus under dexamethasone induction.
- Information by UniProt
- c Myc Interacting protein JPO2 antibody
- CDA7L_HUMAN antibody
- Cdca7l antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CDCA7L/HR1 (green) using ab246958 at 4 μg/ml in ICC/IF.
Anti-CDCA7L/HR1 antibody (ab246958) at 0.4 µg/ml + RT4 (human urinary bladder cancer cell line) whole cell lysate
Predicted band size: 52 kDa
Paraffin-embedded human appendix tissue stained for CDCA7L/HR1 using ab246958 at 1/500 dilution in immunohistochemical analysis.
Anti-CDCA7L/HR1 antibody (ab246958) at 0.4 µg/ml + NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Predicted band size: 52 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246958 has not yet been referenced specifically in any publications.