Overview

  • Product name

  • Description

    Rabbit polyclonal to CDCA7L/HR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CDCA7L/HR1 (C terminal).
    Database link: NP_061189.2

  • Positive control

    • Whole cell lysates from HeLa and 293T cells.
  • General notes

     This product was previously labelled as CDCA7L

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Plays a role in transcriptional regulation as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to the promoter. Plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells. Involved in apoptotic signaling pathways; May act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1.
  • Tissue specificity

    Ubiquitous. Overexpressed in medulloblastoma.
  • Cellular localization

    Cytoplasm. Nucleus. Associates with chromatin. Translocates from cytoplasm to nucleus under dexamethasone induction.
  • Information by UniProt
  • Database links

  • Alternative names

    • c Myc Interacting protein JPO2 antibody
    • CDA7L_HUMAN antibody
    • Cdca7l antibody
    • Cell division cycle-associated 7-like protein antibody
    • DKFZp762L0311 antibody
    • HR1 antibody
    • JPO2 antibody
    • Protein JPO2 antibody
    • R1 antibody
    • RAM2 antibody
    • Transcription factor RAM2 antibody
    see all

Images

  • All lanes : Anti-CDCA7L/HR1 antibody (ab70637) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa
    Additional bands at: 110 kDa, 130 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds
  • Detection of CDCA7L/HR1 by Western Blot of Immunprecipitate. ab70637 at 1µg/ml staining CDCA7L/HR1 in HeLa whole cell lysate immunoprecipitated using ab70637 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:

  • Shen FZ  et al. Cell division cycle associated 7 like predicts unfavorable prognosis and promotes invasion in glioma. Pathol Res Pract 215:50-56 (2019). Read more (PubMed: 30389317) »
  • Ji QK  et al. CDCA7L promotes glioma proliferation by targeting CCND1 and predicts an unfavorable prognosis. Mol Med Rep 20:1149-1156 (2019). Read more (PubMed: 31173217) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Permeabilization
Yes - Triton X 100
Specification
Hela
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 02 2019

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HT1080)
Total protein in input
5 µg
Immuno-precipitation step
Protein A/G
Specification
HT1080

Abcam user community

Verified customer

Submitted Nov 27 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080)
Gel Running Conditions
Non-reduced Denaturing (12)
Loading amount
5 µg
Specification
HT1080
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 21 2018

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