Key features and details
- Rabbit polyclonal to CDCA7L/HR1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CDCA7L/HR1 antibody
See all CDCA7L/HR1 primary antibodies
DescriptionRabbit polyclonal to CDCA7L/HR1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human CDCA7L/HR1 (C terminal).
Database link: NP_061189.2
- Whole cell lysates from HeLa and 293T cells.
This product was previously labelled as CDCA7L
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPlays a role in transcriptional regulation as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to the promoter. Plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells. Involved in apoptotic signaling pathways; May act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1.
Tissue specificityUbiquitous. Overexpressed in medulloblastoma.
Cellular localizationCytoplasm. Nucleus. Associates with chromatin. Translocates from cytoplasm to nucleus under dexamethasone induction.
- Information by UniProt
- c Myc Interacting protein JPO2 antibody
- CDA7L_HUMAN antibody
- Cdca7l antibody
All lanes : Anti-CDCA7L/HR1 antibody (ab70637) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 110 kDa, 130 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Detection of CDCA7L/HR1 by Western Blot of Immunprecipitate. ab70637 at 1µg/ml staining CDCA7L/HR1 in HeLa whole cell lysate immunoprecipitated using ab70637 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.
ab70637 has been referenced in 2 publications.
- Shen FZ et al. Cell division cycle associated 7 like predicts unfavorable prognosis and promotes invasion in glioma. Pathol Res Pract 215:50-56 (2019). PubMed: 30389317
- Ji QK et al. CDCA7L promotes glioma proliferation by targeting CCND1 and predicts an unfavorable prognosis. Mol Med Rep 20:1149-1156 (2019). PubMed: 31173217