Product nameAnti-CDCP1 antibody
See all CDCP1 primary antibodies
DescriptionRabbit polyclonal to CDCP1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human CDCP1 aa 700-800 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Skeletal Muscle tissue lysate and DU145 cell line in IF
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab107165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionMay be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
Tissue specificityHighly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
Sequence similaritiesContains 1 CUB domain.
modificationsPhosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.
Cellular localizationSecreted and Cell membrane. Shedding may also lead to a soluble peptide.
- Information by UniProt
- 9030022E12Rik antibody
- AA409659 antibody
- CD 318 antibody
All lanes : Anti-CDCP1 antibody (ab107165) at 1 µg/ml (Milk Blocked 3%)
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330) with Immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Additional bands at: 27 kDa, 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab107165 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107165, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab107165 has not yet been referenced specifically in any publications.