Recombinant Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free
See all Cdk1 + Cdk2 primary antibodies -
Description
Rabbit monoclonal [EPR17499] to Cdk1 + Cdk2 (phospho T14) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cdk1 + Cdk2 aa 1-100 (phospho T14). The exact sequence is proprietary. Also UniProt - P24941
Database link: P06493 -
General notes
Ab250674 is the carrier-free version of ab183550. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250674 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17499 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab250674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
Target
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Relevance
Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 983 Human
- SwissProt: P06493 Human
- SwissProt: P24941 Human
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Alternative names
- CDC2 antibody
- Cdc2 related protein kinase antibody
- CDC28A antibody
see all
Datasheets and documents
References
ab250674 has not yet been referenced specifically in any publications.
