RecombinantRabMAb

Recombinant Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)

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Western blot - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
  • Immunoprecipitation - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
  • Dot Blot - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
  • Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17499] to Cdk1 + Cdk2 (phospho T14) - BSA and Azide free
  • Suitable for: Dot blot, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free
    See all Cdk1 + Cdk2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17499] to Cdk1 + Cdk2 (phospho T14) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Cdk1 + Cdk2 aa 1-100 (phospho T14). The exact sequence is proprietary. Also UniProt - P24941
    Database link: P06493

  • General notes

    Ab250674 is the carrier-free version of ab183550. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab250674 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab250674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

Target

  • Relevance

    Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.

  • Cellular localization

    Cytoplasmic and Nuclear

  • Database links

    • Alternative names

      • CDC2 antibody
      • Cdc2 related protein kinase antibody
      • CDC28A antibody
      • Cell division cycle 2 antibody
      • Cyclin dependent kinase 2 antibody
      • p33 protein kinase antibody
      • p34 protein kinase antibody
      see all

    Images

    • Western blot - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      Western blot - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      All lanes : Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] (ab183550) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 2mg/ml hydroxyurea for 24 hours.
      Lane 2 : Untreated HeLa whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa


      Exposure time: 30 seconds


      This data was developed using ab183550, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Sequences at the Immunogen region are identical between Cdk1 and Cdk2.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)

      This data was developed using ab183550, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Scattered nuclear and cytoplasmic staining on epithelial cells of Human colon tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      This data was developed using ab183550, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on cancer cells of Human colonic adenocarcinoma tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      This data was developed using ab183550, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on lymphocytes of mouse spleen tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      This data was developed using ab183550, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Cdk1 + Cdk2 (phospho T14) with ab183550 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on lymphocytes of rat spleen tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunoprecipitation - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      Immunoprecipitation - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      This data was developed using ab183550, the same antibody clone in a different buffer formulation.Cdk1 + Cdk2 (phospho T14) was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab183550 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab183550 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.Lane 1 (Input): HeLa whole cell extract 10 µg (Input).Lane 2: ab183550 IP in HeLa whole cell extract.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183550 in HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    • Dot Blot - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      Dot Blot - Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      This data was developed using ab183550, the same antibody clone in a different buffer formulation.Dot blot analysis of Cdk1 + Cdk2 (phospho T14) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab183550 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.Exposure time = 3 minutes
    • Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)
      Anti-Cdk1 + Cdk2 (phospho T14) antibody [EPR17499] - BSA and Azide free (ab250674)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab250674? Please let us know so that we can cite the reference in this datasheet.

    ab250674 has not yet been referenced specifically in any publications.

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