Recombinant Anti-Cdk1-2-3-5 antibody [EP762RY] (ab206314)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP762RY] to Cdk1-2-3-5
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cdk1-2-3-5 antibody [EP762RY]
See all Cdk1-2-3-5 primary antibodies -
Description
Rabbit monoclonal [EP762RY] to Cdk1-2-3-5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and 293T whole cell lysates. 293T transfected with CDK1-2-3-5 expression vector containing a GFP-myc-tag® whole cell lysate. IHC-P: Human lung carcinoma tissue.
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General notes
This product has switched from a hybridoma to recombinant production method on 30th September 2022.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP762RY -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
CDK1 (P06493): Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. CDK2 (P24941): Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. CDK3 (Q00526): Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. CDK5 (Q00535): Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. -
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 1018 Human
- Entrez Gene: 1020 Human
- Entrez Gene: 983 Human
- SwissProt: P06493 Human
- SwissProt: P24941 Human
- SwissProt: Q00526 Human
- SwissProt: Q00535 Human
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Alternative names
- CDC2 antibody
- CDC28A antibody
- CDK1 antibody
see all
Images
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All lanes : Anti-Cdk1-2-3-5 antibody [EP762RY] (ab206314) at 1/5000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure: Lane 1 (180 seconds); Lane 2 (120 seconds).
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All lanes : Anti-Cdk1-2-3-5 antibody [EP762RY] (ab206314) at 1/5000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector containing a GFP-myc-tag® whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with CDK1 expression vector containing a GFP-myc-tag® whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) transfected with CDK2 expression vector containing a GFP-myc-tag® whole cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) transfected with CDK3 expression vector containing GFP-Myc tag® whole cell lysate
Lane 5 : 293T (Human embryonic kidney epithelial cell) transfected with CDK5 expression vector containing GFP-Myc tag® whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Exposure time: 120 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human lung carcinoma labeling Cdk1-2-3-5 with ab206314 at 1/500 (1.95 µg/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab206314 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab206314 has been referenced in 1 publication.
- Pan XW et al. Identification of a novel cancer stem cell subpopulation that promotes progression of human fatal renal cell carcinoma by single-cell RNA-seq analysis. Int J Biol Sci 16:3149-3162 (2020). PubMed: 33162821