Overview

  • Product name

  • Description

    Rabbit polyclonal to CDK1
  • Host species

    Rabbit
  • Specificity

    ab131450 detects endogenous levels of total CDK1 protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, conjugated to KLH, surrounding amino acids 13-17 (G-T-Y-G-V) of Human CDK1 (NP_001163877.1).

  • Positive control

    • Methanol fixed HeLa cells; HeLa and MCF cell extracts; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Tissue specificity

    Isoform 2 is found in breast cancer tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Alternative names

    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Images

  • All lanes : Anti-CDK1 antibody (ab131450) at 1/500 dilution

    Lane 1 : HeLa cell extracts
    Lane 2 : MCF cell extracts

    Predicted band size: 34 kDa

  • Immunofluorescent analysis of methanol fixed HeLa cells labelling CDK1 with ab131450 antibody at a dilution of 1/100.
  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling CDK1 with ab131450 at 1/50 dilution. The image on the right is treated with the synthesized peptide.

References

This product has been referenced in:

  • Wang L  et al. TIPE-2 suppresses growth and aggressiveness of hepatocellular carcinoma cells through downregulation of the phosphoinositide 3-kinase/AKT signaling pathway. Mol Med Rep 17:7017-7026 (2018). Read more (PubMed: 29568863) »
  • Yang YL  et al. pPeOp from Omphalia lapidescens Schroet induces cell cycle arrest and inhibits the migration of MC-4 gastric tumor cells. Oncol Lett 14:533-540 (2017). Read more (PubMed: 28693202) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Botrytis cinerea Tissue lysate - whole (Mycelium of Botrytis cinerea)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
40 µg
Treatment
0.3M hydroxyurea (HU) for 5 hrs
Specification
Mycelium of Botrytis cinerea
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Anna Minz-Dub

Verified customer

Submitted Dec 31 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 02 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 02 2014

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