Western blot abreview for Anti-CDK1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Botrytis cinerea Tissue lysate - whole (Mycelium of Botrytis cinerea)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
40 µg
Treatment
0.3M hydroxyurea (HU) for 5 hrs
Specification
Mycelium of Botrytis cinerea
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG (Jackson ImmunoResearch)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 40 kDa Non-specific: 30, 34 kDa
Positive control
lane 1, UT7, 34 kDa

Dr. Anna Minz-Dub

Verified customer

Submitted Dec 31 2015

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