Product nameAnti-CDK1 antibody
See all CDK1 primary antibodies
DescriptionRabbit polyclonal to CDK1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length Human Cdc2 protein with His tag.
- K562, Jurkat and C6 cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: HEPES, 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
Our Abpromise guarantee covers the use of ab71939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Tissue specificityIsoform 2 is found in breast cancer tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
All lanes : Anti-CDK1 antibody (ab71939) at 1/2000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat T cell lysate
Lane 3 : C6 cell lysate
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 62 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Geng Y et al. Kinase-independent function of E-type cyclins in liver cancer. Proc Natl Acad Sci U S A 115:1015-1020 (2018). Read more (PubMed: 29339491) »
- Otero JJ et al. Cerebellar cortical lamination and foliation require cyclin A2. Dev Biol N/A:N/A (2013). Read more (PubMed: 24184637) »