Product nameAnti-CDK1 antibody [EPR165]
See all CDK1 primary antibodies
DescriptionRabbit monoclonal [EPR165] to CDK1
SpecificityWe have preliminary internal testing data to indicate this antibody doesn’t react with CDK2, CDK3 and CDK5. Please contact us for more information.
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CDK1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P06493
- WB: HeLa, Saos-2, Namalwa, and Jurkat whole cell lysate (ab7899). IHC-P: Human tonsil tissue.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Predicted molecular weight: 34 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Tissue specificityIsoform 2 is found in breast cancer tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
All lanes : Anti-CDK1 antibody [EPR165] (ab133327) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : Namalwa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling CDK1 with ab133327 at 1/500 dilution (0.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). ab150077, a Goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). DAPI nuclear counterstain. Confocal image showing strong nuclear and weakly cytoplasmic staining on HeLa cell line.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling CDK1 with ab133327 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CDK1 with purified ab133327 at 1/130 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This product has been referenced in:
- Kohama Y et al. Regulation of the stability and activity of CDC25A and CDC25B by protein phosphatase PP2A and 14-3-3 binding. Cell Signal 54:10-16 (2019). Read more (PubMed: 30468767) »
- Lin J et al. Exportin-T promotes tumor proliferation and invasion in hepatocellular carcinoma. Mol Carcinog 58:293-304 (2019). Read more (PubMed: 30334580) »