Overview

  • Product name

    Anti-CDK1 antibody - N-terminal
    See all CDK1 primary antibodies
  • Description

    Rabbit polyclonal to CDK1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Chicken, Cow, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human CDK1 (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: P06493

  • Positive control

    • IHC-P: Human breast carcinoma tissue. WB: A549, H1299, NIH/3T3, JC, BCL-1, PC-12, HEK-293T, A431. HeLa, HepG2 and HCT 116 whole cell lysate. ICC/IF: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Tissue specificity

    Isoform 2 is found in breast cancer tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Alternative names

    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Images

  • All lanes : Anti-CDK1 antibody - N-terminal (ab226969) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extracts
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    10% SDS-PAGE

  • MCF7 (human breast adenocarcinoma cell line) cells stained for CDK1 (green) using ab226969 (1/750 dilution) in ICC/IF. MCF7 cells were fixed in ice-cold methanol for 5 minutes. Counterstain: Hoechst 33342 (blue).

  • Paraffin embedded human breast carcinoma tissue stained for CDK1 with ab226969 (1/500 dilution) in immunohistochemical analysis.

  • Anti-CDK1 antibody - N-terminal (ab226969) at 1/5000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    10% SDS PAGE

  • All lanes : Anti-CDK1 antibody - N-terminal (ab226969) at 1/3000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    10% SDS PAGE

  • All lanes : Anti-CDK1 antibody - N-terminal (ab226969) at 1/5000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    10% SDS PAGE

References

ab226969 has not yet been referenced specifically in any publications.

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