Anti-CDK1 antibody [Y100.4] (ab5467)
Key features and details
- Mouse monoclonal [Y100.4] to CDK1
- Suitable for: WB
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG2b
Overview
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Product name
Anti-CDK1 antibody [Y100.4]
See all CDK1 primary antibodies -
Description
Mouse monoclonal [Y100.4] to CDK1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Schizosaccharomyces pombe -
Immunogen
Synthetic C-terminal peptide:
AKRALQQNYLRDFH
(Schizosaccharomyces pombe) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y100.4 -
Myeloma
Sp2 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration.
|
Notes |
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WB
Use at an assay dependent concentration. |
Target
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Function
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
Tissue specificity
Isoform 2 is found in breast cancer tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- SwissProt: P04551 Schizosaccharomyces pombe
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Form
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. -
Alternative names
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab5467 has been referenced in 24 publications.
- Andric V et al. A scaffold lncRNA shapes the mitosis to meiosis switch. Nat Commun 12:770 (2021). PubMed: 33536434
- Pickering M et al. The fission yeast S-phase cyclin Cig2 can drive mitosis. Genetics 217:1-12 (2021). PubMed: 33683349
- Mennie AK et al. LARP7-like protein Pof8 regulates telomerase assembly and poly(A)+TERRA expression in fission yeast. Nat Commun 9:586 (2018). WB ; Schizosaccharomyces pombe . PubMed: 29422503
- Fletcher J et al. Nutrient Limitation Inactivates Mrc1-to-Cds1 Checkpoint Signalling in Schizosaccharomyces pombe. Cells 7:N/A (2018). PubMed: 29473861
- Caspari T et al. The drinking water contaminant dibromoacetonitrile delays G1-S transition and suppresses Chk1 activation at broken replication forks. Sci Rep 7:12730 (2017). PubMed: 28986587