Overview

  • Product name

    Anti-CDK1 antibody [YE324]
    See all CDK1 primary antibodies
  • Description

    Rabbit monoclonal [YE324] to CDK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CDK1 aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Hela cell lysate. IHC-P: Human lymphoma tissue.
  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
    • Tissue specificity

      Isoform 2 is found in breast cancer tissues.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Form

      CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
    • Alternative names

      • Cdc 2 antibody
      • Cdc2 antibody
      • CDC28A antibody
      • CDK 1 antibody
      • CDK1 antibody
      • CDK1_HUMAN antibody
      • CDKN1 antibody
      • CELL CYCLE CONTROLLER CDC2 antibody
      • Cell division control protein 2 antibody
      • Cell division control protein 2 homolog antibody
      • Cell division cycle 2 G1 to S and G2 to M antibody
      • Cell division protein kinase 1 antibody
      • Cell Divsion Cycle 2 Protein antibody
      • Cyclin Dependent Kinase 1 antibody
      • Cyclin-dependent kinase 1 antibody
      • DKFZp686L20222 antibody
      • MGC111195 antibody
      • p34 Cdk1 antibody
      • p34 protein kinase antibody
      • P34CDC2 antibody
      see all

    Images

    • Anti-CDK1 antibody [YE324] (ab32094) at 1/2000 dilution + Hela cell lysate

      Predicted band size: 34 kDa
      Observed band size: 34 kDa

    References

    This product has been referenced in:

    • Strauss B  et al. Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis. J Cell Biol 217:179-193 (2018). Read more (PubMed: 29074707) »
    • Zöllner SK  et al. Inhibition of the oncogenic fusion protein EWS-FLI1 causes G2-M cell cycle arrest and enhanced vincristine sensitivity in Ewing's sarcoma. Sci Signal 10:N/A (2017). Read more (PubMed: 28974650) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab32094.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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