Key features and details
- Rabbit polyclonal to CDK1 (phospho T14)
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CDK1 (phospho T14) antibody
See all CDK1 primary antibodies
DescriptionRabbit polyclonal to CDK1 (phospho T14)
SpecificityDetects endogenous levels of CDC2 only when phosphorylated at threonine 14.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phosphopeptide derived from human Cdc2 around the phosphorylation site of threonine 14 (E-G-TP-Y-G).
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab58509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 34 kDa).|
|ICC/IF||1/100 - 1/500.|
FunctionPlays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Tissue specificityIsoform 2 is found in breast cancer tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
FormCDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM for 30 mins, using CDK1 (phospho T14) Antibody (ab58509). The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of COS7 cells using ab58509 at 1/1000 dilution.
All lanes : Anti-CDK1 (phospho T14) antibody (ab58509) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells,
treated with Forskolin (40nM, 30mins)
Lane 2 : Extracts from HepG2 cells,
treated with Forskolin (40nM, 30mins), plus immunising peptide
Predicted band size: 34 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab58509 has been referenced in 1 publication.
- Chow JP & Poon RY The CDK1 inhibitory kinase MYT1 in DNA damage checkpoint recovery. Oncogene : (2012). PubMed: 23146904