Anti-CDK1 (phospho T161) antibody (ab194874)
Key features and details
- Rabbit polyclonal to CDK1 (phospho T161)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CDK1 (phospho T161) antibody
See all CDK1 primary antibodies -
Description
Rabbit polyclonal to CDK1 (phospho T161) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CDK1 (phospho T161). The exact sequence is proprietary.
Database link: P06493 -
Positive control
- HeLa cell lysate and Human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 34 kDa.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 34 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
Tissue specificity
Isoform 2 is found in breast cancer tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 983 Human
- Omim: 116940 Human
- SwissProt: P06493 Human
- Unigene: 334562 Human
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Form
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. -
Alternative names
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
Images
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All lanes : Anti-CDK1 (phospho T161) antibody (ab194874) at 1/500 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with UV
Predicted band size: 34 kDa -
Immunohistochemical staining of Human breast carcinoma tissue labeling CDK1 (phospho T161) using ab194874 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab194874 has been referenced in 1 publication.
- Mehl JL et al. Blockage of lamin-A/C loss diminishes the pro-inflammatory macrophage response. iScience 25:105528 (2022). PubMed: 36465100