Anti-CDK1 (phospho T161) antibody (ab47329)


  • Product name
    Anti-CDK1 (phospho T161) antibody
    See all CDK1 primary antibodies
  • Description
    Rabbit polyclonal to CDK1 (phospho T161)
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of Threonine161 (V-Y-TP-H-E).

  • Positive control
    • COLO cells, K562 cells, human breast carcinoma tissue.
  • General notes

    This antibody detects CDC2 only when phosphorylated at Threonine 161.



Our Abpromise guarantee covers the use of ab47329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use at an assay dependent concentration.


  • Function
    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Tissue specificity
    Isoform 2 is found in breast cancer tissues.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Form
    CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Alternative names
    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all


  • ab47329 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of synthesized phospho-peptide using Alkaline phosphatase Goat anti rabbit IgG (H+L) secondary antibody.
  • All lanes : Anti-CDK1 (phospho T161) antibody (ab47329) at 1/500 dilution

    Lane 1 : COLO cell extract preincubated with immunogenic phospho-peptide. with phosphopeptide at 1 µg/ml
    Lane 2 : COLO cell extract, untreated.
    Lane 3 : K362 cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • ICC/IF image of ab47329 stained MCF7 cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47329, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Roseweir AK  et al. Phosphorylation of androgen receptors at serine 515 is a potential prognostic marker for triple negative breast cancer. Oncotarget 8:37172-37185 (2017). IHC-P ; Human . Read more (PubMed: 28415597) »
  • Wang J  et al. Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients. Oncol Lett 9:1266-1272 (2015). IHC ; Mouse . Read more (PubMed: 25663895) »

See all 4 Publications for this product

Customer reviews and Q&As

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Thank you for contacting us. Isotype controls are the primary antibodies that do not have specificity for any target. These can be used as negative control and are helpful in determining the background staining due to secondary antibodies. By compa...

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Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa cells)
HeLa cells
Yes - 0.05% Triton X 100 in PBS for 5 min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - other (HeLa cells)
Loading amount
20 µg
HeLa cells
Gel Running Conditions
Reduced Denaturing (8% Tris Glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Andrea McCollum

Verified customer

Submitted Aug 20 2010


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