Overview

  • Product name

    Anti-CDK1 (phospho Y15) antibody
    See all CDK1 primary antibodies
  • Description

    Rabbit polyclonal to CDK1 (phospho Y15)
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for CDK1.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CDK1 aa 5-54.
    Database link: P06493

  • Positive control

    • HepG2 cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab47594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
ELISA 1/10000.

Target

  • Function

    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
  • Tissue specificity

    Isoform 2 is found in breast cancer tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
  • Alternative names

    • Cdc 2 antibody
    • Cdc2 antibody
    • CDC28A antibody
    • CDK 1 antibody
    • CDK1 antibody
    • CDK1_HUMAN antibody
    • CDKN1 antibody
    • CELL CYCLE CONTROLLER CDC2 antibody
    • Cell division control protein 2 antibody
    • Cell division control protein 2 homolog antibody
    • Cell division cycle 2 G1 to S and G2 to M antibody
    • Cell division protein kinase 1 antibody
    • Cell Divsion Cycle 2 Protein antibody
    • Cyclin Dependent Kinase 1 antibody
    • Cyclin-dependent kinase 1 antibody
    • DKFZp686L20222 antibody
    • MGC111195 antibody
    • p34 Cdk1 antibody
    • p34 protein kinase antibody
    • P34CDC2 antibody
    see all

Images

  • Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + COS7 cells

    Predicted band size: 34 kDa

  • Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + HepG2 cell extract

    Predicted band size: 34 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/1000 dilution

    Lane 1 : untreated HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with nocodazole (500ng/mL) for 16 h

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : polyclonal goat HRP conjugated at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa
    Observed band size: 34 kDa


    Exposure time: 1 minute


    Blocking: 5% milk for 1 hour at 25°C.

    See Abreview

References

This product has been referenced in:

  • Sun Z  et al. miR-181c-5p mediates simulated microgravity-induced impaired osteoblast proliferation by promoting cell cycle arrested in the G2 phase. J Cell Mol Med 23:3302-3316 (2019). Read more (PubMed: 30761733) »
  • Duan Y  et al. Wee1 kinase inhibitor MK-1775 induces apoptosis of acute lymphoblastic leukemia cells and enhances the efficacy of doxorubicin involving downregulation of Notch pathway. Oncol Lett 16:5473-5481 (2018). Read more (PubMed: 30250620) »
See all 22 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Treatment
1 - untreated, 2- nocodazole 500 ng/ml 16 h
Gel Running Conditions
Reduced Denaturing (8% Tris Glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 13 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X 100 in PBS for 5 min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 06 2010

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