Anti-CDK1 (phospho Y15) antibody (ab47594)
Key features and details
- Rabbit polyclonal to CDK1 (phospho Y15)
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
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Overview
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Product name
Anti-CDK1 (phospho Y15) antibody
See all CDK1 primary antibodies -
Description
Rabbit polyclonal to CDK1 (phospho Y15) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Human CDK1 aa 1-100 (phospho Y15).
Database link: P06493 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
Tissue specificity
Isoform 2 is found in breast cancer tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 983 Human
- Omim: 116940 Human
- SwissProt: P06493 Human
- Unigene: 334562 Human
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Form
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. -
Alternative names
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
Images
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Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + COS-7 cells (African green monkey kidney fibroblast-like)
Predicted band size: 34 kDa -
Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + HepG2 cell extract
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/1000 dilution
Lane 1 : untreated HeLa cell lysate
Lane 2 : HeLa cell lysate treated with nocodazole (500ng/mL) for 16 h
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : polyclonal goat HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minuteBlocking: 5% milk for 1 hour at 25°C.
Datasheets and documents
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SDS download
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Datasheet download
References (38)
ab47594 has been referenced in 38 publications.
- Liu JCY et al. Mechanism and function of DNA replication-independent DNA-protein crosslink repair via the SUMO-RNF4 pathway. EMBO J 40:e107413 (2021). PubMed: 34346517
- Zhang X et al. Treatment of Melanoma by Nano-conjugate-Delivered Wee1 siRNA. Mol Pharm 18:3387-3400 (2021). PubMed: 34375118
- Liu J et al. Knockdown of MAPK14 inhibits the proliferation and migration of clear cell renal cell carcinoma by downregulating the expression of CDC25B. Cancer Med 9:1183-1195 (2020). PubMed: 31856414
- Gray S et al. Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner. Cell Rep 32:107858 (2020). PubMed: 32640224
- Guo X et al. RNA demethylase ALKBH5 prevents pancreatic cancer progression by posttranscriptional activation of PER1 in an m6A-YTHDF2-dependent manner. Mol Cancer 19:91 (2020). PubMed: 32429928