Overview

  • Product name

  • Description

    Rabbit polyclonal to CDK12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CDK12 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.

  • Positive control

    • Mouse liver tissue lysate.
  • General notes

    Protein previously labeled as CrkRS.

Properties

Applications

Our Abpromise guarantee covers the use of ab37914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 145 kDa (predicted molecular weight: 164 kDa).

Target

  • Function

    Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation at Thr-893 increases kinase activity.
  • Cellular localization

    Nucleus. Nucleus speckle. Colocalized with nuclear speckles throughout interphase.
  • Information by UniProt
  • Database links

  • Alternative names

    • arginine/serine-rich antibody
    • Cdc2 related kinase arginine/serine rich antibody
    • CDC2 related protein kinase 7 antibody
    • Cdc2-related kinase antibody
    • CDC2-related protein kinase 7 antibody
    • Cdk12 antibody
    • CDK12_HUMAN antibody
    • Cell division cycle 2 related protein kinase 7 antibody
    • Cell division cycle 2-related protein kinase 7 antibody
    • Cell division protein kinase 12 antibody
    • CRK7 antibody
    • CRKR antibody
    • CrkRS antibody
    • Cyclin-dependent kinase 12 antibody
    • EC 2.7.11.22 antibody
    • hCDK12 antibody
    • kiaa0904 antibody
    • Pksc antibody
    see all

Images

References

This product has been referenced in:

  • Natrajan R  et al. Characterization of the genomic features and expressed fusion genes in micropapillary carcinomas of the breast. J Pathol 232:553-65 (2014). Read more (PubMed: 24395524) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 15 2010

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