Key features and details
- Rabbit polyclonal to Cdk2
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Cdk2 antibody
See all CDK2 primary antibodies
DescriptionRabbit polyclonal to Cdk2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow, Chinese hamster
- IHC-P: Human liver, liver cancer, breast cancer and colorectal cancer tissues. WB: HeLa, HepG2 and K562 cell lysates; Pig liver lysate; Recombinant human Cdk2 protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232753 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 34 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- Cdc2 related protein kinase antibody
- cdc2-related protein kinase antibody
- CDC28 antibody
All lanes : Anti-Cdk2 antibody (ab232753) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : Pig liver lysate
Predicted band size: 34 kDa
Paraffin-embedded human liver tissue stained for Cdk2 using ab232753 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human liver cancer tissue stained for Cdk2 using ab232753 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human breast cancer tissue stained for Cdk2 using ab232753 at 20 µg/ml in immunohistochemical analysis, followed by HRP-Linked Goat Anti-Rabbit IgG Polyclonal Antibody at 2 µg/ml. DAB staining.
Paraffin-embedded human colorectal cancer tissue stained for Cdk2 using ab232753 at 20 µg/ml in immunohistochemical analysis, followed by HRP-Linked Goat Anti-Rabbit IgG Polyclonal Antibody at 2 µg/ml. DAB staining.
Anti-Cdk2 antibody (ab232753) at 2 µg/ml + Recombinant human Cdk2 protein
Predicted band size: 34 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232753 has not yet been referenced specifically in any publications.