Anti-Cdk2 antibody (ab235941)

Paraffin-embedded human colon cancer tissue stained for Cdk2 using ab235941 at 1/800 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Cdk2 using ab235941 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

Paraffin-embedded human tonsil tissue stained for Cdk2 using ab235941 at 1/800 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Cdk2 was immunoprecipitated from 0.5 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with ab235941 at 1/200 dilution. 

Lane 1: Control rabbit IgG IP (1 μg) in HeLa whole cell lysate.

Lane 2: ab235941 IP in HeLa whole cell extract.

Lane 3: HeLa whole cell lysate 10 μg (Input).

For western blotting, an HRP-conjugated light chain specific antibody was used as the secondary antibody at 1/50000 dilution.