Overview

  • Product name

  • Description

    Rabbit polyclonal to Cdk2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chinese hamster, Syrian hamster
  • Immunogen

    Recombinant full length protein corresponding to Human Cdk2 aa 1-298.
    Sequence:

    MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIR EISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIP LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLAR AFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRR ALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSK VVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL


    Database link: P24941

  • Positive control

    • WB: K562, HEK-293, HeLa, Jurkat and HepG2 whole cell lysates. IHC-P: Human tonsil and colon cancer tissues. ICC/IF: HepG2 cells. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab235941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 34 kDa.
IP 1/200 - 1/2000.
IHC-P 1/500 - 1/1000.
ICC/IF 1/50 - 1/500.

Target

Images

  • All lanes : Anti-Cdk2 antibody (ab235941) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 34 kDa

  • Paraffin-embedded human colon cancer tissue stained for Cdk2 using ab235941 at 1/800 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Cdk2 using ab235941 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human tonsil tissue stained for Cdk2 using ab235941 at 1/800 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Cdk2 was immunoprecipitated from 0.5 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with ab235941 at 1/200 dilution. 

    Lane 1: Control rabbit IgG IP (1 μg) in HeLa whole cell lysate.

    Lane 2: ab235941 IP in HeLa whole cell extract.

    Lane 3: HeLa whole cell lysate 10 μg (Input).

    For western blotting, an HRP-conjugated light chain specific antibody was used as the secondary antibody at 1/50000 dilution.

References

ab235941 has not yet been referenced specifically in any publications.

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