Overview

  • Product name

  • Description

    Rabbit polyclonal to Cdk2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Chinese hamster, Syrian hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Cdk2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab71917.)

  • Positive control

    • This antibody gave a positive signal in the following Lysates: HeLa Whole Cell, Rat Thymus Tissue, HeLa Whole Cell - Bleomycin Treated (20U/ml), Jurkat Whole Cell, Y79 Whole Cell

Properties

Applications

Our Abpromise guarantee covers the use of ab64669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

Images

  • Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)

    Lanes 2, 4 and 6: CDK2 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Green signal from target – ab64669 observed at 34 kDa

    Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa

    Lanes 5 and 6: Merged (red and green) signal

    ab64669 was shown to react with CDK2 when CDK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK2 knockout samples were subjected to SDS-PAGE. ab64669 and ab8226 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776
    secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-Cdk2 antibody (ab64669) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Thymus (Rat) Tissue Lysate
    Lane 4 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Zheng X  et al. MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts. Braz J Med Biol Res 51:e7574 (2018). Read more (PubMed: 30365725) »
  • Kim JJ  et al. WSB1 overcomes oncogene-induced senescence by targeting ATM for degradation. Cell Res 27:274-293 (2017). Read more (PubMed: 27958289) »
See all 2 Publications for this product

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