Product nameAnti-Cdk2 antibody
See all Cdk2 primary antibodies
DescriptionRabbit polyclonal to Cdk2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Dog, Chinese hamster, Syrian hamster
- This antibody gave a positive signal in the following Lysates: HeLa Whole Cell, Rat Thymus Tissue, HeLa Whole Cell - Bleomycin Treated (20U/ml), Jurkat Whole Cell, Y79 Whole Cell
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
FunctionInvolved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- Cdc2 related protein kinase antibody
- cdc2-related protein kinase antibody
- CDC28 antibody
Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 4 and 6: CDK2 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Green signal from target – ab64669 observed at 34 kDa
Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
ab64669 was shown to react with CDK2 when CDK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK2 knockout samples were subjected to SDS-PAGE. ab64669 and ab8226 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776
secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-Cdk2 antibody (ab64669) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lane 4 : HeLa Whole Cell Lysate - Bleomycin Treated (20U/ml)
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Zheng X et al. MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts. Braz J Med Biol Res 51:e7574 (2018). Read more (PubMed: 30365725) »
- Kim JJ et al. WSB1 overcomes oncogene-induced senescence by targeting ATM for degradation. Cell Res 27:274-293 (2017). Read more (PubMed: 27958289) »