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  1. Link

    cdk2-antibody-c-terminal-ab224771.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Cdks
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Anti-Cdk2 antibody - C-terminal (ab224771)

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  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody - C-terminal (ab224771)
  • Western blot - Anti-Cdk2 antibody - C-terminal (ab224771)

Key features and details

  • Rabbit polyclonal to Cdk2 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Protein
Product image
Recombinant Human Cdk2 protein (ab111646)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cdk2 antibody - C-terminal
    See all CDK2 primary antibodies
  • Description

    Rabbit polyclonal to Cdk2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cdk2 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P24941

  • Positive control

    • WB: HeLa whole cell lysate (ab150035). IHC-P: Human tonsil tissue.
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdks
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Cdks
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Cdks

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Cdk2 protein (ab111646)
  • Related Products

    • Recombinant Human Cdk2 protein (ab125664)
    • Recombinant Human Cdk2 protein (ab126917)

Applications

Our Abpromise guarantee covers the use of ab224771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25. Predicted molecular weight: 34 kDa.
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer before commencing with IHC staining protocol.

Target

  • Function

    Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession P24941 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1017 Human
    • Omim: 116953 Human
    • SwissProt: P24941 Human
    • Unigene: 19192 Human
    • Unigene: 689624 Human
    • Alternative names

      • Cdc2 related protein kinase antibody
      • cdc2-related protein kinase antibody
      • CDC28 antibody
      • CDC2A antibody
      • Cdk 2 antibody
      • CDK1 antibody
      • CDK2 antibody
      • CDK2_HUMAN antibody
      • CDKN2 antibody
      • Cell devision kinase 2 antibody
      • Cell division protein kinase 2 antibody
      • Cyclin dependent kinase 2 antibody
      • cyclin dependent kinase 2-alpha antibody
      • Cyclin-dependent kinase 2 antibody
      • kinase Cdc2 antibody
      • MPF antibody
      • p33 protein kinase antibody
      • p33(CDK2) antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody - C-terminal (ab224771)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody - C-terminal (ab224771)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for Cdk2 using ab224771 at 1/100 dilution in immunohistochemical analysis.

    • Western blot - Anti-Cdk2 antibody - C-terminal (ab224771)
      Western blot - Anti-Cdk2 antibody - C-terminal (ab224771)
      Anti-Cdk2 antibody - C-terminal (ab224771) at 1/25 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

      Predicted band size: 34 kDa

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab224771? Please let us know so that we can cite the reference in this datasheet.

    ab224771 has not yet been referenced specifically in any publications.

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