Recombinant Anti-Cdk2 antibody [SP80] (ab101682)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP80] to CDK2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Cdk2 antibody [SP80]
See all CDK2 primary antibodies -
Description
Rabbit monoclonal [SP80] to CDK2 -
Host species
Rabbit -
Specificity
Based on the immunogen sequence, we do not expect the antibody to cross-react with other CDK family members. No cross-reactivity testing has been performed.
-
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cdk2 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P24941 -
Positive control
- IHC-P: Human tonsil, mouse and rat spleen
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP80 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/50. Predicted molecular weight: 34 kDa. for 1 hour at RT.
|
|
IHC-P |
1/100. Incubate for 30 minutes at RT. Antigen retrieval is recommended: boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min.
|
Notes |
---|
WB
1/50. Predicted molecular weight: 34 kDa. for 1 hour at RT. |
IHC-P
1/100. Incubate for 30 minutes at RT. Antigen retrieval is recommended: boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min. |
Target
-
Function
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
-
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
- SwissProt: Q63699 Rat
- Unigene: 19192 Human
see all -
Alternative names
- Cdc2 related protein kinase antibody
- cdc2-related protein kinase antibody
- CDC28 antibody
see all
Images
-
All lanes : Anti-Cdk2 antibody [SP80] (ab101682) at 1/200 dilution
Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : CDK2 knockout MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaWestern blot: Anti-CDK2 antibody [SP80] (ab101682) staining at 1/200 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab101682 was shown to bind specifically to CDK2. A band was observed at 34 kDa in wild-type MCF7 cell lysates with no signal observed at this size in CDK2 knockout cell line ab282628. To generate this image, wild-type and CDK2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Cdk2 with ab101682 at 1/50 dilution (2.20 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101682 for 30 mins at room temperature. -
Anti-Cdk2 antibody [SP80] (ab101682) at 1/100 dilution + lysate prepared from HeLa cells
Predicted band size: 34 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling Cdk2 with ab101682 at 1/50 dilution (2.20 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101682 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling Cdk2 with ab101682 at 1/50 dilution (2.20 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the mouse spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101682 for 30 mins at room temperature. -
Immunohistochemical analysis of Cdk2 expression in formalin-fixed, paraffin-embedded Human tonsil using ab101682 at 1/100 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab101682 has been referenced in 4 publications.
- Chen C et al. ASF1b is a novel prognostic predictor associated with cell cycle signaling pathway in gastric cancer. J Cancer 13:1985-2000 (2022). PubMed: 35399734
- Zhang Y et al. LncRNA-CCDC144NL-AS1 Promotes the Development of Hepatocellular Carcinoma by Inducing WDR5 Expression via Sponging miR-940. J Hepatocell Carcinoma 8:333-348 (2021). PubMed: 33977095
- Ji W et al. Combined Androgen receptor blockade overcomes the resistance of breast cancer cells to palbociclib. Int J Biol Sci 15:522-532 (2019). PubMed: 30745839
- Qi H et al. Activation of AMPK Attenuated Cardiac Fibrosis by Inhibiting CDK2 via p21/p27 and miR-29 Family Pathways in Rats. Mol Ther Nucleic Acids 8:277-290 (2017). PubMed: 28918029