Recombinant Anti-CDK2 (phospho T14) antibody [EP2234Y] - BSA and Azide free (ab239860)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2234Y] to CDK2 (phospho T14) - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Chinese hamster
Related conjugates and formulations
Overview
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Product name
Anti-CDK2 (phospho T14) antibody [EP2234Y] - BSA and Azide free
See all CDK2 primary antibodies -
Description
Rabbit monoclonal [EP2234Y] to CDK2 (phospho T14) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects Cdk2 phosphorylated on Threonine 14.
The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human, Chinese hamster -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa,C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours, C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours, CHO-S (Chinese hamster ovary epithelial cell) whole cell lysates. IHC: Human ovarian carcinoma, mouse and rat stomach tissues.
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General notes
ab239860 is the carrier-free version of ab68265.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2234Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster. |
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 100689039 Chinese hamster
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: O55076 Chinese hamster
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
see all -
Alternative names
- Cdc2 related protein kinase antibody
- cdc2-related protein kinase antibody
- CDC28 antibody
see all
Images
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All lanes : Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution
Lane 1 : CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate
Lane 2 : CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa -
This data was developed using ab239860, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling CDK2 with Purified 239860 at 1:1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
Lane 1 : Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution (Purified)
Lanes 2-6 : Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution
Lane 1 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours whole cell lysate
Lane 3 : C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 4 : Untreated C2C12 (Mouse myoblasts myoblast) whole cell lysate
Lane 5 : C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours whole cell lysate
Lane 6 : C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa -
This data was developed using 239860, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling CDK2 with Purified 239860 at 1:1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
This data was developed using 239860, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling CDK2 with Purified 239860 at 1:1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
All lanes : Anti-CDK2 (phospho T14) antibody [EP2234Y] - BSA and Azide free (ab239860) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 3mM hydroxyurea for 24 hours whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 3mM hydroxyurea for 24 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab239860 has not yet been referenced specifically in any publications.