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Synthetic peptide within Human Cdk5 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab40773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Lanes 1 - 4: Merged signal (red and green). Green - ab40773 observed at 33 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab40773 was shown to specifically react with Cdk5 in wild-type HAP1 cells as signal was lost in CDK5 knockout cells. Wild-type and CDK5 knockout samples were subjected to SDS-PAGE. Ab40773 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
ab40773 staining Cdk5 in the SH-SY5Y cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/300). a150077 was used as the secondary antibody (1/500) and nuclei stained with DAPI.
Immunohistochemical analysis of frozen PFA-fixed chicken midbrain tissue cyrosection, labelling Cdk5 with ab40773 at a dilution of 1/200 incubated for 16 hours at 4°C in 0.1% PBS Triton X and 3% BSA. Blocking was with 3% BSA incubated for 1 hour at 21°C. Secondary was a goat anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/1000.
Paraffin-embedded human brain tissue stained for Cdk5 with ab40773 at 1/100 dilution.
Flow cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Cdk5 with ab40773 (pink). Negative control Rabbit IgG (green).
ab40773 at 1/50 immunoprecipitating Cdk5 in HeLa whole cell lysate.
For western blotting, the primary antibody was used at a dilution of 1/1000 and a HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"