The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500 - 1/15000. Detects a band of approximately 238 kDa (predicted molecular weight: 215 kDa).
Use at 3 µg/mg of lysate.
Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends.
Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Involvement in disease
Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3) [MIM:604804]. A disorder defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
Phosphorylated in vitro by CDK5.
Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasm. Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends.
CDK5 regulatory subunit associated protein 2 antibody
CDK5 regulatory subunit-associated protein 2 antibody
centrosomal protein 215 kDa antibody
Centrosome-associated protein 215 antibody
Western blot - Anti-CDK5RAP2 antibody (ab70213)
All lanes : Anti-CDK5RAP2 antibody (ab70213) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from NIH3T3 cells at 50 µg
Detection of Human CDK5RAP2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 1/4 of IP loaded) using affinity purified rabbit anti-CDK5RAP2 antibody
ab70213 at 3 µg/mg of lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note: bands between 55 and 71 kDa represent the IgG chains.