Overview

  • Product name

  • Description

    Rabbit polyclonal to CDK5RAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1843 and the C-terminus (residue 1893) of Human CDK5RAP2 (NP_060719.4)

  • Positive control

    • HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab86340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    ICC/IF: 1/100 - 1/500. Fix with formaldehyde of methanol. Do not fix with acetone. Permeabilization with Triton-X 100 recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with MAPRE1, it may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends.
    • Tissue specificity

      Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Involvement in disease

      Defects in CDK5RAP2 are the cause of microcephaly primary type 3 (MCPH3) [MIM:604804]. A disorder defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
    • Post-translational
      modifications

      Phosphorylated in vitro by CDK5.
    • Cellular localization

      Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasm. Found in the pericentriolar region adhering to the surface of the centrosome and in the region of the centrosomal appendages. Localizes to microtubule plus ends.
    • Information by UniProt
    • Database links

    • Alternative names

      • C48 antibody
      • CDK5 activator-binding protein C48 antibody
      • CDK5 regulatory subunit associated protein 2 antibody
      • CDK5 regulatory subunit-associated protein 2 antibody
      • CDK5RAP2 antibody
      • centrosomal protein 215 kDa antibody
      • Centrosome-associated protein 215 antibody
      • CEP215 antibody
      • CK5P2_HUMAN antibody
      • MCPH3 antibody
      see all

    Images

    • ab86340 at 1/250 dilution staining CDK5RAP2 in NBF-fixed asynchronous HeLa cells by Imunofluorescence.
      Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1/100.
    • ab86340 at 1/250 dilution staining CDK5RAP2 in NBF-fixed asynchronous HeLa cells by Imunofluorescence.
      Detection: Anti-rabbit IgG-FITC conjugated used at a dilution of 1/100.

    References

    This product has been referenced in:

    • Munkley J  et al. The androgen receptor controls expression of the cancer-associated sTn antigen and cell adhesion through induction of ST6GalNAc1 in prostate cancer. Oncotarget 6:34358-74 (2015). Read more (PubMed: 26452038) »
    • Astuti D  et al. Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility. Nat Genet 44:277-84 (2012). Read more (PubMed: 22306653) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Gel Running Conditions
    Reduced Denaturing (5-20% gradient)
    Loading amount
    50 µg
    Treatment
    1 µg/ml plasmid DNA transfection for 24 hrs
    Specification
    HEK293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Aug 15 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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