Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-CDK5RAP3 antibody [EPR11293]
See all CDK5RAP3 primary antibodies -
DescriptionRabbit monoclonal [EPR11293] to CDK5RAP3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to residues in Human CDK5RAP3 (UniProt Q96JB5).
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Positive control
- 293T, HeLa, U87-MG and A431 cell lysates; U87-MG cells; Immunoprecipitation pellet from 293T cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR11293
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab168353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 57 kDa. | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. |
Target
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FunctionPotential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.
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Tissue specificityUbiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.
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Sequence similaritiesBelongs to the CDK5RAP3 family.
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Post-translational
modificationsPhosphorylated in vitro by CDK5. - Information by UniProt
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Database links
- Entrez Gene: 80279 Human
- Entrez Gene: 80280 Mouse
- Entrez Gene: 80278 Rat
- Omim: 608202 Human
- SwissProt: Q96JB5 Human
- SwissProt: Q99LM2 Mouse
- SwissProt: Q9JLH7 Rat
- Unigene: 20157 Human
see all -
Alternative names
- C53 antibody
- CDK5 activator binding protein C53 antibody
- CDK5 activator-binding protein C53 antibody
see all
Images
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![Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.com/ps/datasheet/images/168/ab168353/CDK5RAP3-Primary-antibodies-ab168353-1.jpg)
Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)All lanes : Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.com/ps/datasheet/images/168/ab168353/CDK5RAP3-Primary-antibodies-ab168353-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)Immunofluorescent analysis of U87-MG cells labeling CDK5RAP3 with ab168353 at 1/50 dilution. -
![Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.com/ps/datasheet/images/168/ab168353/CDK5RAP3-Primary-antibodies-ab168353-3.jpg)
Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution + immunoprecipitation pellet from 293T cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 57 kDa
Protocols
References
ab168353 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"