Recombinant Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
![Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.com/ps/products/168/ab168353/Images/ab168353-166625-anti-cdk5rap3-antibody-epr11293-western-blot.jpg "Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11293] to CDK5RAP3
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
-
Product name
Anti-CDK5RAP3 antibody [EPR11293]
See all CDK5RAP3 primary antibodies -
Description
Rabbit monoclonal [EPR11293] to CDK5RAP3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human CDK5RAP3.
-
Positive control
- 293T, HeLa, U87-MG and A431 cell lysates; U87-MG cells; Immunoprecipitation pellet from 293T cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11293 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
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Isotype control
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Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 57 kDa.
|
|
| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 57 kDa. |
|
ICC/IF
1/50 - 1/100. |
Target
-
Function
Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1. -
Tissue specificity
Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta. -
Sequence similarities
Belongs to the CDK5RAP3 family. -
Post-translational
modificationsPhosphorylated in vitro by CDK5. - Information by UniProt
-
Database links
- Entrez Gene: 80279 Human
- Entrez Gene: 80280 Mouse
- Entrez Gene: 80278 Rat
- Omim: 608202 Human
- SwissProt: Q96JB5 Human
- SwissProt: Q99LM2 Mouse
- SwissProt: Q9JLH7 Rat
- Unigene: 20157 Human
see all -
Alternative names
- C53 antibody
- CDK5 activator binding protein C53 antibody
- CDK5 activator-binding protein C53 antibody
see all
Images
-
Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)All lanes : Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.com/ps/products/168/ab168353/Images/ab168353-166624-anti-cdk5rap3-antibody-epr11293-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)Immunofluorescent analysis of U87-MG cells labeling CDK5RAP3 with ab168353 at 1/50 dilution. -
![Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.com/ps/products/168/ab168353/Images/ab168353-166623-anti-cdk5rap3-antibody-epr11293-western-blot.jpg)
Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution + immunoprecipitation pellet from 293T cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 57 kDa -
![Anti-CDK5RAP3 antibody [EPR11293] (ab168353)](https://www.abcam.com/ps/products/168/ab168353/Images/ab168353-4-benefits-of-recombinant-antibodies.png)
Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
Protocols
Datasheets and documents
References (1)
ab168353 has been referenced in 1 publication.
- Chen QY et al. CDK5RAP3 Inhibits the Translocation of MCM6 to Influence the Prognosis in Gastric Cancer. J Cancer 10:4488-4498 (2019). PubMed: 31528213
