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Cell Biology Cell Cycle Kinases/Phosphatases Cdks
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RecombinantRabMAb

Recombinant Anti-CDK5RAP3 antibody [EPR11293] (ab168353)

  • Datasheet
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Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
  • Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
  • Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
  • Anti-CDK5RAP3 antibody [EPR11293] (ab168353)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11293] to CDK5RAP3
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-CDK5RAP3 antibody [EPR11293]
    See all CDK5RAP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR11293] to CDK5RAP3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human CDK5RAP3.

  • Positive control

    • 293T, HeLa, U87-MG and A431 cell lysates; U87-MG cells; Immunoprecipitation pellet from 293T cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11293
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Cdks
    • Cell Biology
    • Apoptosis
    • Intracellular
    • NFkB
    • RelA (p65)
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • NFkB
    • RelA (p65)

Associated products

  • Alternative Versions

    • Anti-CDK5RAP3 antibody [EPR11293] - BSA and Azide free (ab249450)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human CDK5RAP3 protein (ab201715)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab168353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 57 kDa.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 57 kDa.
ICC/IF
1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.
    • Tissue specificity

      Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.
    • Sequence similarities

      Belongs to the CDK5RAP3 family.
    • Post-translational
      modifications

      Phosphorylated in vitro by CDK5.
    • Target information above from: UniProt accession Q96JB5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 80279 Human
      • Entrez Gene: 80280 Mouse
      • Entrez Gene: 80278 Rat
      • Omim: 608202 Human
      • SwissProt: Q96JB5 Human
      • SwissProt: Q99LM2 Mouse
      • SwissProt: Q9JLH7 Rat
      • Unigene: 20157 Human
      • Unigene: 28297 Mouse
      • Unigene: 3727 Rat
      see all
    • Alternative names

      • C53 antibody
      • CDK5 activator binding protein C53 antibody
      • CDK5 activator-binding protein C53 antibody
      • CDK5 regulatory subunit associated protein 3 antibody
      • CDK5 regulatory subunit-associated protein 3 antibody
      • Cdk5rap3 antibody
      • CK5P3_HUMAN antibody
      • HSF 27 antibody
      • HSF27 antibody
      • IC53 antibody
      • Ischemic heart CDK5 activator binding protein C53 antibody
      • LXXLL/leucine zipper containing ARFbinding protein antibody
      • LZAP antibody
      • MST016 antibody
      • OK/SW cl.114 antibody
      • PP1553 antibody
      • Protein HSF-27 antibody
      see all

    Images

    • Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      All lanes : Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Immunofluorescent analysis of U87-MG cells labeling CDK5RAP3 with ab168353 at 1/50 dilution.
    • Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution + immunoprecipitation pellet from 293T cell lysate

      Secondary
      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

      Predicted band size: 57 kDa

    • Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Anti-CDK5RAP3 antibody [EPR11293] (ab168353)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab168353? Please let us know so that we can cite the reference in this datasheet.

    ab168353 has been referenced in 1 publication.

    • Chen QY  et al. CDK5RAP3 Inhibits the Translocation of MCM6 to Influence the Prognosis in Gastric Cancer. J Cancer 10:4488-4498 (2019). PubMed: 31528213

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab168353.
    Please use the links above to contact us or submit feedback about this product.

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