Recombinant
RabMAb

Recombinant Anti-CDK5RAP3 antibody [EPR11293] (ab168353)

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Overview

  • Product name

    Anti-CDK5RAP3 antibody [EPR11293]
    See all CDK5RAP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11293] to CDK5RAP3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human CDK5RAP3.

  • Positive control

    • 293T, HeLa, U87-MG and A431 cell lysates; U87-MG cells; Immunoprecipitation pellet from 293T cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab168353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 57 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.

    • Tissue specificity

      Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.

    • Sequence similarities

      Belongs to the CDK5RAP3 family.

    • Post-translational
      modifications

      Phosphorylated in vitro by CDK5.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • C53 antibody
      • CDK5 activator binding protein C53 antibody
      • CDK5 activator-binding protein C53 antibody
      • CDK5 regulatory subunit associated protein 3 antibody
      • CDK5 regulatory subunit-associated protein 3 antibody
      • Cdk5rap3 antibody
      • CK5P3_HUMAN antibody
      • HSF 27 antibody
      • HSF27 antibody
      • IC53 antibody
      • Ischemic heart CDK5 activator binding protein C53 antibody
      • LXXLL/leucine zipper containing ARFbinding protein antibody
      • LZAP antibody
      • MST016 antibody
      • OK/SW cl.114 antibody
      • PP1553 antibody
      • Protein HSF-27 antibody
      see all

    Images

    • Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      All lanes : Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Immunocytochemistry/ Immunofluorescence - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Immunofluorescent analysis of U87-MG cells labeling CDK5RAP3 with ab168353 at 1/50 dilution.
    • Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Western blot - Anti-CDK5RAP3 antibody [EPR11293] (ab168353)
      Anti-CDK5RAP3 antibody [EPR11293] (ab168353) at 1/1000 dilution + immunoprecipitation pellet from 293T cell lysate

      Secondary
      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

      Predicted band size: 57 kDa

    References

    ab168353 has not yet been referenced specifically in any publications.

    Publishing research using ab168353? Please let us know so that we can cite the reference in this datasheet.

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