Overview

  • Product name

    Anti-CDK5RAP3 antibody [EPR11294(B)]
    See all CDK5RAP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR11294(B)] to CDK5RAP3
  • Host species

    Rabbit
  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CDK5RAP3 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96JB5

  • Positive control

    • WB: Jurkat, HeLa, A431, CTLL-2, EL4, H9 and 293T cell lysates. IHC-P: Human pancreas and stomach tissue. Flow Cyt: HEK-293T cells. IP: CDK5RAP3 IP in HEK-293T cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.
    • Tissue specificity

      Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.
    • Sequence similarities

      Belongs to the CDK5RAP3 family.
    • Post-translational
      modifications

      Phosphorylated in vitro by CDK5.
    • Information by UniProt
    • Database links

    • Alternative names

      • C53 antibody
      • CDK5 activator binding protein C53 antibody
      • CDK5 activator-binding protein C53 antibody
      • CDK5 regulatory subunit associated protein 3 antibody
      • CDK5 regulatory subunit-associated protein 3 antibody
      • Cdk5rap3 antibody
      • CK5P3_HUMAN antibody
      • HSF 27 antibody
      • HSF27 antibody
      • IC53 antibody
      • Ischemic heart CDK5 activator binding protein C53 antibody
      • LXXLL/leucine zipper containing ARFbinding protein antibody
      • LZAP antibody
      • MST016 antibody
      • OK/SW cl.114 antibody
      • PP1553 antibody
      • Protein HSF-27 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CDK5RAP3 with purified ab157203 at 1/50 dilution (2.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/2000 dilution (Purified)

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 57 kDa
      Observed band size: 57 kDa

    • All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution (Purified)

      Lane 1 : CTLL-2 (Mouse T lymphocyte) whole cell lysates
      Lane 2 : EL4 (Mouse lymphoma T lymphocyte) whole cell lysates
      Lane 3 : H9 (Human lymphoma T lymphocyte) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 57 kDa
      Observed band size: 57 kDa

    • ab157203 (purified) at 1/20 dilution (1ug) immunoprecipitating CDK5RAP3 in HEK-293 whole cell lysate. HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
      Lane 2 (+): ab157203 & HEK-293 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab157203 in HEK-293 whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDK5RAP3 with purified ab157203 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue, labeling CDK5RAP3 using ab157203 (unpurified) at a 1/50 dilution.

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot analysis of Immunoprecipitation pellet from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate showing CDK5RAP3, using ab157203 (unpurified) at 1/10 dilution.

    • All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution (unpurified)

      Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
      Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 57 kDa

    • Flow cytometry analysis of permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling CDK5RAP3 (red), using ab157203 (unpurified) at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    This product has been referenced in:

    • Zheng CH  et al. CDK5RAP3 suppresses Wnt/ß-catenin signaling by inhibiting AKT phosphorylation in gastric cancer. J Exp Clin Cancer Res 37:59 (2018). Read more (PubMed: 29540196) »
    • Lin JX  et al. Low expression of CDK5RAP3 and DDRGK1 indicates a poor prognosis in patients with gastric cancer. World J Gastroenterol 24:3898-3907 (2018). Read more (PubMed: 30228783) »
    See all 2 Publications for this product

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