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Rabbit monoclonal [EPR11294(B)] to CDK5RAP3
, Flow Cytmore detailsUnsuitable for:
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human CDK5RAP3 (Uniprot: Q96JB5)
- Jurkat, HeLa, A431 and 293T cell lysates; pancreas tissue; 293T cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
||1/10 - 1/100.
||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC/IF.
Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.
Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.
Belongs to the CDK5RAP3 family.
Phosphorylated in vitro by CDK5.
Information by UniProt
- C53 antibody
- CDK5 activator binding protein C53 antibody
- CDK5 activator-binding protein C53 antibody
Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)
Western blot analysis of Immunoprecipitation pellet from 293T cell lysate showing CDK5RAP3, using ab157203 at 1/10 dilution.
Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)
All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling CDK5RAP3 using ab157203 at a 1/50 dilution.
Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)
Flow cytometry analysis of permeabilized 293T cells labeling CDK5RAP3 (red), using ab157203 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"