Overview

  • Product name
    Anti-CDK5RAP3 antibody [EPR11294(B)]
    See all CDK5RAP3 primary antibodies
  • Description
    Rabbit monoclonal [EPR11294(B)] to CDK5RAP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human CDK5RAP3 (Uniprot: Q96JB5)

  • Positive control
    • Jurkat, HeLa, A431 and 293T cell lysates; pancreas tissue; 293T cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.
    • Tissue specificity
      Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.
    • Sequence similarities
      Belongs to the CDK5RAP3 family.
    • Post-translational
      modifications
      Phosphorylated in vitro by CDK5.
    • Information by UniProt
    • Database links
    • Alternative names
      • C53 antibody
      • CDK5 activator binding protein C53 antibody
      • CDK5 activator-binding protein C53 antibody
      • CDK5 regulatory subunit associated protein 3 antibody
      • CDK5 regulatory subunit-associated protein 3 antibody
      • Cdk5rap3 antibody
      • CK5P3_HUMAN antibody
      • HSF 27 antibody
      • HSF27 antibody
      • IC53 antibody
      • Ischemic heart CDK5 activator binding protein C53 antibody
      • LXXLL/leucine zipper containing ARFbinding protein antibody
      • LZAP antibody
      • MST016 antibody
      • OK/SW cl.114 antibody
      • PP1553 antibody
      • Protein HSF-27 antibody
      see all

    Images

    • Western blot analysis of Immunoprecipitation pellet from 293T cell lysate showing CDK5RAP3, using ab157203 at 1/10 dilution.
    • All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 57 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling CDK5RAP3 using ab157203 at a 1/50 dilution.
    • Flow cytometry analysis of permeabilized 293T cells labeling CDK5RAP3 (red), using ab157203 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    This product has been referenced in:
    • Zheng CH  et al. CDK5RAP3 suppresses Wnt/ß-catenin signaling by inhibiting AKT phosphorylation in gastric cancer. J Exp Clin Cancer Res 37:59 (2018). Read more (PubMed: 29540196) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157203.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up