RecombinantRabMAb

Recombinant Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
  • Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
  • Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
  • Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
  • Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
  • Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11294(B)] to CDK5RAP3 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free
    See all CDK5RAP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11294(B)] to CDK5RAP3 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested applications

    Suitable for: IHC-P, IP, WB, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human CDK5RAP3 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96JB5

  • Positive control

    • IHC-P: Human pancreas tissue, Human stomach tissue. Flow Cyt: HEK-293T cells. IP: CDK5RAP3 IP in HEK-293T cells.

  • General notes

    ab242399 is the carrier-free version of ab157203 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab242399 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab242399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

 See IHC antigen retrieval protocols.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
Flow Cyt 1/10.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.

    • Tissue specificity

      Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.

    • Sequence similarities

      Belongs to the CDK5RAP3 family.

    • Post-translational
      modifications

      Phosphorylated in vitro by CDK5.
    • Information by UniProt

    • Database links

      • Alternative names

        • C53 antibody
        • CDK5 activator binding protein C53 antibody
        • CDK5 activator-binding protein C53 antibody
        • CDK5 regulatory subunit associated protein 3 antibody
        • CDK5 regulatory subunit-associated protein 3 antibody
        • Cdk5rap3 antibody
        • CK5P3_HUMAN antibody
        • HSF 27 antibody
        • HSF27 antibody
        • IC53 antibody
        • Ischemic heart CDK5 activator binding protein C53 antibody
        • LXXLL/leucine zipper containing ARFbinding protein antibody
        • LZAP antibody
        • MST016 antibody
        • OK/SW cl.114 antibody
        • PP1553 antibody
        • Protein HSF-27 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CDK5RAP3 with purified ab157203 at 1/50 dilution (2.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157203).

      • Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
        Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

        ab157203 (purified) at 1/20 dilution (1ug) immunoprecipitating CDK5RAP3 in HEK-293 whole cell lysate. HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
        Lane 2 (+): ab157203 & HEK-293 whole cell lysate
        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab157203 in HEK-293 whole cell lysate
        For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
        Blocking and diluting buffer: 5% NFDM/TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157203).

      • Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
        Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

        Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDK5RAP3 with purified ab157203 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157203).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue, labeling CDK5RAP3 using ab157203 (unpurified) at a 1/50 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol, and sodium azide (ab157203)

        Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
        Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

        Western blot analysis of Immunoprecipitation pellet from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate showing CDK5RAP3, using ab157203 (unpurified) at 1/10 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol, and sodium azide (ab157203)

      • Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
        Flow Cytometry - Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

        Flow cytometry analysis of permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling CDK5RAP3 (red), using ab157203 (unpurified) at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green).

        This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol, and sodium azide (ab157203)

      • Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
        Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab242399? Please let us know so that we can cite the reference in this datasheet.

      ab242399 has not yet been referenced specifically in any publications.

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