Recombinant Anti-Cdk6 antibody [98D] (ab241554)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [98D] to Cdk6
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cdk6 antibody [98D]
See all Cdk6 primary antibodies -
Description
Mouse monoclonal [98D] to Cdk6 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Mouse Cdk6 aa 1-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q00534 -
Positive control
- WB: HeLa, Jurkat, K562, RAW 264.7 and PC-12 whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
98D -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.518 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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Notes |
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WB
Use a concentration of 0.518 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 1021 Human
- Entrez Gene: 12571 Mouse
- Entrez Gene: 114483 Rat
- Omim: 603368 Human
- SwissProt: Q00534 Human
- SwissProt: Q64261 Mouse
- Unigene: 119882 Human
- Unigene: 31672 Mouse
see all -
Alternative names
- CDK 6 antibody
- CDK6 antibody
- CDK6_HUMAN antibody
see all
Images
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All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 1/2000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CDK6 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab241554 observed at 38 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab241554 was shown to react with Cdk6 in HeLa wild-type cells in Western blot with loss of signal observed in CDK6 knockout cell line ab266059 (CDK6 knockout cell lysate ab257088). Wild-type HeLa and CDK6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab241554 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/ml
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 3 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate
Lane 4 : Saos-2 (human osteosarcoma epithelial), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Negative control: PANC-1, Saos-2. (PMID: 8114739).
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All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/ml
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab241554 has been referenced in 4 publications.
- Guo X et al. High Expression of PIGC Predicts Unfavorable Survival in Hepatocellular Carcinoma. J Hepatocell Carcinoma 8:211-222 (2021). PubMed: 33854986
- Wang Y et al. Hsa_circ_0002062 Promotes the Proliferation of Pulmonary Artery Smooth Muscle Cells by Regulating the Hsa-miR-942-5p/CDK6 Signaling Pathway. Front Genet 12:673229 (2021). PubMed: 34322152
- Sheng W et al. Upregulation of Linc00284 Promotes Lung Cancer Progression by Regulating the miR-205-3p/c-Met Axis. Front Genet 12:694571 (2021). PubMed: 34616424
- Wu Q et al. Salvianolic Acid Alleviated Blood-Brain Barrier Permeability in Spontaneously Hypertensive Rats by Inhibiting Apoptosis in Pericytes via P53 and the Ras/Raf/MEK/ERK Pathway. Drug Des Devel Ther 14:1523-1534 (2020). PubMed: 32368011