Overview

  • Product name

  • Description

    Rabbit polyclonal to Cdk6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 35-309 of Human Cdk6 (UniProt Q00534).

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human SW480 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab151247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-Cdk6 antibody (ab151247) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 37 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PPIH with ab151247 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue labeling Cdk6 with ab151247 at 1/500 dilution.

References

This product has been referenced in:

  • Tormo E  et al. The miRNA-449 family mediates doxorubicin resistance in triple-negative breast cancer by regulating cell cycle factors. Sci Rep 9:5316 (2019). Read more (PubMed: 30926829) »
  • Bai Y  et al. Golgi integral membrane protein 4 manipulates cellular proliferation, apoptosis, and cell cycle in human head and neck cancer. Biosci Rep 38:N/A (2018). Read more (PubMed: 30068697) »
See all 11 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mammary tumor)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
Mammary tumor
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 20 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial OVCAR cell line)
Gel Running Conditions
Reduced Denaturing (10 % SDS-PAGE)
Loading amount
100 µg
Treatment
Perifosine 24 hr
Specification
Epithelial OVCAR cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 27 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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